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AB27648

HRP Anti-Nitrotyrosine antibody

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(2 Publications)

Goat Polyclonal Nitrotyrosine antibody - conjugated to HRP. Suitable for ELISA, WB and reacts with Chemical samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Nitrotyrosine.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by nitrated protein-Sepharose™ affinity column.
Storage buffer
pH: 6.5 - 7.4 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.58% Sodium chloride, 0.134% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.
Biological function summary

Nitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.

Pathways

Proteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.

Nitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Nitrotyrosine
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Current protocols in toxicology Chapter 17:Unit 17.15 PubMed22511115

2012

High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Jin,Richard C Zangar

Talanta 81:1662-9 PubMed20441955

2010

Sensitive immunoassays of nitrated fibrinogen in human biofluids.

Applications

ELISA

Species

Unspecified reactive species

Zhiwen Tang,Hong Wu,Dan Du,Jun Wang,Hua Wang,Wei-jun Qian,Diana J Bigelow,Joel G Pounds,Richard D Smith,Yuehe Lin
View all publications
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