JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB205470

HRP Anti-non-muscle Myosin IIA antibody [EPR8965]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MYH9 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.

View Alternative Names

Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9

2 Images
Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (AB205470)
  • WB

Lab

Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (AB205470)

ab205470 was shown to specifically react with non-muscle Myosin IIA in wild-type HAP1 cells as signal was lost in MYH9 (non-muscle Myosin IIA) knockout cells. Wild-type and MYH9 (non-muscle Myosin IIA) knockout samples were subjected to SDS-PAGE. ab205470 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

MYH9 (non-muscle Myosin IIA) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 227 kDa

Observed band size: 230 kDa

false

Exposure time: 20s

Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (AB205470)
  • WB

Lab

Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (AB205470)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205470 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

Kidney (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 227 kDa

Observed band size: 230 kDa

true

Exposure time: 15s

  • Unconjugated

    Anti-non-muscle Myosin IIA antibody [EPR8965]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-non-muscle Myosin IIA antibody [EPR8965]

  • 578 PE

    PE Anti-non-muscle Myosin IIA antibody [EPR8965]

  • Carrier free

    Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-non-muscle Myosin IIA antibody [EPR8965]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8965

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Non-muscle Myosin IIA also known as Myosin Heavy Chain 9 or Myosin-9 is a motor protein that enables cellular mechanical tasks such as actin filament cross-linking and contraction. It has a molecular mass of approximately 226 kDa. Myosin IIA is expressed in a variety of cell types including epithelial cells fibroblasts and leukocytes where it contributes to various cellular activities. Myosin staining often reveals its localization at the leading edge of migrating cells revealing its involvement in cell movement.
Biological function summary

Non-muscle Myosin IIA plays an essential role in maintaining cell shape and promoting cell division. It combines with other myosin proteins into a complex often in conjunction with actin filaments. This myosin protein group further facilitates processes like cytokinesis and cellular migration. Myosin IIA's complex interactions allow cells to adapt to different mechanical stresses ensuring that cells can move and divide properly within the tissue architecture.

Pathways

Non-muscle Myosin IIA participates in both the RhoA/ROCK pathway and the actin cytoskeleton regulation pathway. Through these pathways Myosin IIA interacts with other key proteins such as Rho-associated protein kinase to mediate several cellular functions like cell shape changes and motility. These interactions allow Myosin IIA to efficiently transmit mechanical signals and respond to extracellular stimuli.

Research links non-muscle Myosin IIA with specific conditions like cancer invasion and cardiovascular disorders. Abnormal function or expression of Myosin IIA can contribute to altered cell motility resulting in cancer metastasis. Additionally mutations or disruptions in Myosin IIA can associate with conditions such as thrombocytopenia. Proteins like integrins interact with Myosin IIA in these disease states highlighting their critical roles in pathological settings.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed : 16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed : 20052411).. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.
See full target information Myosin-9
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB241068

Anti-non-muscle Myosin IIA antibody

Western blot - Anti-non-muscle Myosin IIA antibody (AB241068)

  • 0
  • 0
Primary Antibodies

AB75590

Anti-non-muscle Myosin IIA antibody

KO Validated

Western blot - Anti-non-muscle Myosin IIA antibody (AB75590)

  • 5
  • 5

Complementary Products

Primary Antibodies

AB190573

Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

  • 5
  • 3
Primary Antibodies

AB282114

Alexa Fluor® 594 Anti-CD163 antibody [EPR19518]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD163 antibody [EPR19518] (AB282114)

  • 1
  • 1
Primary Antibodies

AB224942

Alexa Fluor® 647 Anti-p53 antibody [E26]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p53 antibody [E26] (AB224942)

  • 0
  • 1
Primary Antibodies

AB206278

Alexa Fluor® 647 Anti-Actin antibody [EPR16769] - Cytoskeleton Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Actin antibody [EPR16769] - Cytoskeleton Marker (AB206278)

  • 0
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com