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AB201995

HRP Anti-O-Linked N-Acetylglucosamine antibody [RL2]

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(5 Publications)

Mouse Monoclonal O-Linked N-Acetylglucosamine antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 5 publications.
1 Images
Western blot - HRP Anti-O-Linked N-Acetylglucosamine antibody [RL2] (AB201995)
  • WB

Lab

Western blot - HRP Anti-O-Linked N-Acetylglucosamine antibody [RL2] (AB201995)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201995 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab201995) at 1/1000 dilution

Lane 1:

Jurkat treated with 0 uM PugNAc at 20 µg

Lane 2:

Jurkat treated with 4 mM glucosamine and 50 uM PugNAc (3 hours) at 20 µg

Observed band size: 100 kDa,65 kDa

false

Exposure time: 2min

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-O-Linked N-Acetylglucosamine antibody [RL2]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RL2

Isotype

IgG1

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

O-Linked N-Acetylglucosamine (O-GlcNAc) is a post-translational modification involving the addition of a single N-acetylglucosamine moiety to the serine or threonine residues of nuclear and cytoplasmic proteins. This dynamic modification is sometimes referred to as O-GlcNAcylation. The molecular mass of the O-GlcNAc group itself is approximately 203 Da. This modification is expressed widely across various tissues notably in the brain and pancreas. O-GlcNAc plays a critical role in regulating protein function stability and interaction.
Biological function summary

O-GlcNAc modifies proteins influencing cellular processes such as transcription signal transduction and stress response. It is involved in the regulation of transcription factors like Sp1 and NF-kB and is associated with the O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) enzymes which respectively add and remove the GlcNAc group. O-GlcNAc functions in a manner similar to phosphorylation often competing with it on the same or adjacent serine/threonine sites. This modification is not part of a permanent protein complex but dynamically modulates protein interactions and activity.

Pathways

This modification plays a fundamental role in pathways related to nutrient sensing and insulin signaling. It modulates proteins such as insulin receptor substrate 1 (IRS1) and Akt to link nutrient status to cellular responses. The hexosamine biosynthetic pathway (HBP) is an important pathway in which O-GlcNAc is synthesized. Through these pathways it impacts cellular signaling and metabolism influencing processes like cellular growth and apoptosis.

O-GlcNAc modification connects to conditions like diabetes and Alzheimer's disease. Elevated O-GlcNAc levels contribute to insulin resistance a hallmark of type 2 diabetes through interaction with proteins such as IRS1 and Akt. In Alzheimer's O-GlcNAc modifies tau protein reducing its phosphorylation and aggregation therefore potentially affecting neurofibrillary tangle formation. These connections underline the importance of O-GlcNAc in the pathophysiology of these diseases and highlight its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information O-Linked N-Acetylglucosamine
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e30526 PubMed38737268

2024

Changes in transcriptomic landscape with macronutrients intake switch are independent from O-GlcNAcylation levels in heart throughout postnatal development in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Persello,Thomas Dupas,Amandine Vergnaud,Angélique Blangy-Letheule,Virginie Aillerie,Angélique Erraud,Yannick Guilloux,Manon Denis,Benjamin Lauzier

International journal of molecular sciences 25: PubMed38732103

2024

Overexpression of Fatty Acid Synthase Upregulates Glutamine-Fructose-6-Phosphate Transaminase 1 and O-Linked N-Acetylglucosamine Transferase to Increase O-GlcNAc Protein Glycosylation and Promote Colorectal Cancer Growth.

Applications

Unspecified application

Species

Unspecified reactive species

James Drury,Mariah E Geisen,Josiane Weber Tessmann,Piotr G Rychahou,Courtney O Kelson,Daheng He,Chi Wang,B Mark Evers,Yekaterina Y Zaytseva

International journal of molecular sciences 25: PubMed38542290

2024

NButGT Reinforces the Beneficial Effects of Epinephrine on Cardiac Mitochondrial Respiration, Lactatemia and Cardiac Output in Experimental Anaphylactic Shock.

Applications

Unspecified application

Species

Unspecified reactive species

Walid Oulehri,Antoine Persello,Angelique Blangy-Letheule,Charles Tacquard,Bertrand Rozec,Anne-Laure Charles,Bernard Geny,Benjamin Lauzier,Paul Michel Mertes,Olivier Collange

Life science alliance 5: PubMed34667079

2021

PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokang Wang,Wojciech Rosikiewicz,Yurii Sedkov,Tanner Martinez,Baranda S Hansen,Patrick Schreiner,Jesper Christensen,Beisi Xu,Shondra M Pruett-Miller,Kristian Helin,Hans-Martin Herz

Cancer letters 517:24-34 PubMed34052331

2021

Normalizing glucose levels reconfigures the mammary tumor immune and metabolic microenvironment and decreases metastatic seeding.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Allah M Alsheikh,Brandon J Metge,Chae-Myeong Ha,Dominique C Hinshaw,Mateus S V Mota,Sarah C Kammerud,Tshering Lama-Sherpa,Noha Sharafeldin,Adam R Wende,Rajeev S Samant,Lalita A Shevde
View all publications
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