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Rabbit Recombinant Monoclonal p27 KIP 1 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - HRP Anti-p27 KIP 1 antibody [Y236] (AB194235), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p27 KIP 1 antibody [Y236] (AB194235), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p27 KIP 1 antibody [Y236] (AB194235), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

HRP

Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/70 - 1/500

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

1 products for Alternative Version

Target data

Function

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Alternative names

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, p27, KIP1

Recommended products

Rabbit Recombinant Monoclonal p27 KIP 1 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples.

Alternative names

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, p27, KIP1

Key facts

Isotype

IgG

Conjugation

HRP

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

Y236

Purification technique

Affinity purification Protein A

Dissociation constant

2.1 x 10-11 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.

Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Associated diseases and disorders

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - HRP Anti-p27 KIP 1 antibody [Y236] (ab194235), expandable thumbnail

    Western blot - HRP Anti-p27 KIP 1 antibody [Y236] (ab194235)

    ab194235 was shown to recognize p27 KIP 1 in wild-type HAP1 cells as signal was lost at the expected MW in CDKN1B (p27 KIP 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CDKN1B (p27 KIP 1) knockout samples were subjected to SDS-PAGE. ab194235 and Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    All lanes: Western blot - HRP Anti-p27 KIP 1 antibody [Y236] (ab194235) at 1/5000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: CDKN1B (p27 KIP 1) knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 22 kDa

    Exposure time: 2min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p27 KIP 1 antibody [Y236] (ab194235), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p27 KIP 1 antibody [Y236] (ab194235)

    IHC image of p27 KIP 1 staining in a section of formalin-fixed paraffin-embedded human colon adenocarcinoma*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194235 at 1/70 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p27 KIP 1 antibody [Y236] (ab194235), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p27 KIP 1 antibody [Y236] (ab194235)

    IHC image of p27 KIP 1 staining in a section of formalin-fixed paraffin-embedded human colon adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab194235 at 1μg/ml. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and manual) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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