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AB181737

HRP Anti-Papain antibody

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(1 Publication)

Goat Polyclonal Papain antibody - conjugated to HRP. Suitable for WB and reacts with Papaya samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Papaya Papain.

View Alternative Names

Papain, Papaya proteinase I, PPI

1 Images
Western blot - HRP Anti-Papain antibody (AB181737)
  • WB

Supplier Data

Western blot - HRP Anti-Papain antibody (AB181737)

Block : MB-070 for 30 min at Room temperature.
Predicted/Observed size : 23.4 kDa, >25 kDa for Papain.

All lanes:

Western blot - HRP Anti-Papain antibody (ab181737) at 1/1000 dilution

All lanes:

Papain at 0.05 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Papaya

Applications

WB

applications

Immunogen

Native Full Length Protein corresponding to Papaya Papain.

P00784

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Papaya": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Papain also known as papaya proteinase I is a cysteine protease enzyme derived from the papaya plant. It exhibits a molecular weight of approximately 23.4 kDa. Papain is synthesized primarily in the fruit of the papaya and its enzyme activity depends on the presence of thiol groups. This protease is popular in various industrial applications due to its ability to cleave a wide range of peptide bonds facilitating protein breakdown. Disulfide bonds within papain help stabilize its structure allowing efficient substrate digestion under different conditions.
Biological function summary

Papain functions as a proteolytic enzyme aiding in the hydrolysis of proteins into peptides and amino acids. It is not part of a complex but rather operates independently to perform its function. Papain's broad proteolytic activity makes it effective in breaking down tough protein structures in organisms enhancing nutrient availability. The enzyme's activity finds use in meat tenderizing and has a role in certain cellular processes such as protein catabolism.

Pathways

The enzyme plays a role in dietary protein digestion and degradation pathways. It interacts with other proteases like ficin and bromelain working synergistically to facilitate the denaturation and digestion of proteins. These proteases including papain contribute significantly to metabolic processes that involve protein turnover and nitrogen metabolism in various organisms. Papain substrates often include proteins that require further degradation for metabolic purposes.

Papain's relevance is notable concerning inflammation and wound healing. Studies suggest that papain's proteolytic properties help in removing dead tissue and promoting new tissue formation benefiting chronic wounds and ulcers. Papain also shows potential in managing inflammatory conditions where its degradation of specific protein substrates can minimize inflammatory responses. Proteins like elastase may connect to papain's activity as it mediates tissue regeneration and inflammation resolution.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cysteine proteinase with a high level of diversity in substrate specificity, an amino acid bearing a large hydrophobic side chain at the P2 position is preferred.
See full target information Papain
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1414 PubMed34676007

2021

Aligned nanofiber nerve conduits inhibit alpha smooth muscle actin expression and collagen proliferation by suppressing TGF-β1/SMAD signaling in traumatic neuromas.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglun Yao,Xijie Zhou,Weidong Weng,Keshav Poonit,Chao Sun,Hede Yan
View all publications
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Product promise

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