AB202837

HRP Anti-PAX5 antibody [EPR3730(2)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal PAX5 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5

1 Images

Lab

Western blot - HRP Anti-PAX5 antibody [EPR3730(2)] (AB202837)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202837 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-PAX5 antibody [EPR3730(2)] (ab202837) at 1/5000 dilution

All lanes:

Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 42 kDa

Observed band size: 48 kDa

true

Exposure time: 1min

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PAX5 antibody [EPR3730(2)]
578 PE

PE Anti-PAX5 antibody [EPR3730(2)]
Biotin

Biotin Anti-PAX5 antibody [EPR3730(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PAX5 antibody [EPR3730(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-PAX5 antibody [EPR3730(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PAX5 antibody [EPR3730(2)]
660 APC

APC Anti-PAX5 antibody [EPR3730(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-PAX5 antibody [EPR3730(2)]
Unconjugated

Anti-PAX5 antibody [EPR3730(2)]
Carrier free

Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3730(2)

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.

Biological function summary

PAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.

Pathways

PAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.

PAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed : 10811620, PubMed : 27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed : 10811620, PubMed : 27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed : 32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed : 32612238).. (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed : 31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed : 23678172).

See full target information PAX5

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 10: PubMed35728874

2022

Antibody-based redirection of universal Fabrack-CAR T cells selectively kill antigen bearing tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chiu Kuo,Cheng-Fu Kuo,Kurt Jenkins,Alfur Fu-Hsin Hung,Wen-Chung Chang,Miso Park,Brenda Aguilar,Renate Starr,Jonathan Hibbard,Christine Brown,John C Williams

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-PAX5 antibody [EPR3730(2)]

Western blot - HRP Anti-PAX5 antibody [EPR3730(2)] (AB202837)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise