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Rabbit Recombinant Monoclonal PCNA antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PCNA antibody [EPR3821] (AB193965), expandable thumbnail
  • Western blot - HRP Anti-PCNA antibody [EPR3821] (AB193965), expandable thumbnail

Publications

  • BMC biology 21:1012023
    Insights into the formation and diversification of a novel chiropteran wing membrane from embryonic development.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Neal Anthwal,Daniel J Urban,Alexa Sadier,Risa Takenaka,Simon Spiro,Nancy Simmons,Richard R Behringer,Chris J Cretekos,John J Rasweiler,Karen E Sears
    PubMed 37143038
  • Journal of anatomy 242:224-2342022
    Mechanisms driving vestibular lamina formation and opening in the mouse.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Tengyang Qiu,Abigail S Tucker
    PubMed 36181694

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

HRP

Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

    5 products for Alternative Version

      Target data

      Function

      Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

      Alternative names

      Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

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      Rabbit Recombinant Monoclonal PCNA antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

      Alternative names

      Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

      Key facts

      Isotype

      IgG

      Conjugation

      HRP

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR3821

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

      Biological function summary

      This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

      Pathways

      PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

      Associated diseases and disorders

      PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      2 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PCNA antibody [EPR3821] (ab193965), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PCNA antibody [EPR3821] (ab193965)

        IHC image of PCNA staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193965 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

        *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

      • Western blot - HRP Anti-PCNA antibody [EPR3821] (ab193965), expandable thumbnail

        Western blot - HRP Anti-PCNA antibody [EPR3821] (ab193965)

        This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193965 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

        All lanes: Western blot - HRP Anti-PCNA antibody [EPR3821] (ab193965) at 1/5000 dilution

        Lane 1: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035) at 10 µg

        Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

        Lane 3: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

        Lane 4: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 29 kDa

        Observed band size: 29 kDa

        Exposure time: 30s

      Downloads

      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com