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AB324173

HRP Anti-PDE4B antibody [EPR11830]

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Rabbit Recombinant Monoclonal PDE4B3 antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

DPDE4, PDE4B, PDE32, cAMP-specific phosphodiesterase 4B

2 Images
Western blot - HRP Anti-PDE4B antibody [EPR11830] (AB324173)
  • WB

Supplier Data

Western blot - HRP Anti-PDE4B antibody [EPR11830] (AB324173)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - HRP Anti-PDE4B antibody [EPR11830] (ab324173) at 1/1000 dilution

All lanes:

Human hippocampus tissue lysate at 20 µg

Observed band size: 83 kDa

false

Exposure time: 15s

Western blot - HRP Anti-PDE4B antibody [EPR11830] (AB324173)
  • WB

Supplier Data

Western blot - HRP Anti-PDE4B antibody [EPR11830] (AB324173)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - HRP Anti-PDE4B antibody [EPR11830] (ab324173) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Observed band size: 83 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11830

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Mouse, Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 4B (PDE4B) also known as PDE4B2 is an enzyme that specifically breaks down cyclic adenosine monophosphate (cAMP) to AMP an important process in cellular signaling. It belongs to the PDE4 family which is characterized by its ability to regulate the concentration of cAMP within cells. PDE4B has a molecular mass of approximately 75 kDa and is frequently expressed in immune cells including monocytes and neutrophils as well as in the brain.
Biological function summary

PDE4B plays a significant role in modulating intracellular signaling by controlling the levels of cAMP a second messenger involved in various cellular processes. As part of the larger PDE4 family complex PDE4B assists in fine-tuning immune responses and inflammatory processes. This enzyme modulates the activity of protein kinase A (PKA) impacting processes such as cell proliferation differentiation and apoptosis.

Pathways

PDE4B operates within the cAMP signaling pathway which is essential for transducing extracellular signals into cellular responses. This pathway involves interaction with other proteins such as PKA and CREB (cAMP response element-binding protein) which are critical for regulating gene expression and cellular functions. PDE4B's ability to modulate this pathway makes it fundamental in controlling the dynamics of cellular signaling.

PDE4B has been associated with inflammatory diseases such as asthma and chronic obstructive pulmonary disease (COPD). Its involvement in regulating cAMP levels affects the inflammatory response linking it to the pathogenesis of these conditions. Additionally PDE4B influences the activity of inflammatory mediators and cytokines contributing to disease progression and symptoms. For example PDE4 inhibitors can modulate these pathways presenting potential therapeutic opportunities for managing related inflammatory disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 15260978). May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
See full target information PDE4B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com