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AB197956

HRP Anti-PDHA1 antibody [9H9AF5]

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(2 Publications)

Mouse Monoclonal PDHA1 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PHE1A, PDHA1, PDHE1-A type I

1 Images
Western blot - HRP Anti-PDHA1 antibody [9H9AF5] (AB197956)
  • WB

Lab

Western blot - HRP Anti-PDHA1 antibody [9H9AF5] (AB197956)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197956 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-PDHA1 antibody [9H9AF5] (ab197956) at 1/3000 dilution

Lane 1:

Human heart tissue lysate - mitochondrial extract (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-mitochondrial-extract-ab110337'>ab110337</a>) at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

true

Exposure time: 8min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9H9AF5

Isotype

IgG1

Light chain type

kappa

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
ab110330 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.
Biological function summary

PDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.

Pathways

PDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.

Mutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
See full target information PDHA1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:18078-18097 PubMed37563971

2023

UBE2S promotes malignant properties via VHL/HIF-1α and VHL/JAK2/STAT3 signaling pathways and decreases sensitivity to sorafenib in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Wu,Xiangjie Xu,Shasha Wu,Weiwei Shi,Guang Zhang,Yin Cao,Zhongxia Wang,Junhua Wu,Chunping Jiang

Biochimica et biophysica acta. Molecular basis of disease 1866:165964 PubMed32920119

2020

P-cadherin induces anoikis-resistance of matrix-detached breast cancer cells by promoting pentose phosphate pathway and decreasing oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Sousa,Joana Pereira,Ricardo Marques,Luís F Grilo,Susana P Pereira,Vilma A Sardão,Fernando Schmitt,Paulo J Oliveira,Joana Paredes
View all publications
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