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AB201733

HRP Anti-PDIA6 antibody [EPR10132(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal PDIA6 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ERP5, P5, TXNDC7, PDIA6, Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, ER protein 5, ERp5

1 Images
Western blot - HRP Anti-PDIA6 antibody [EPR10132(B)] (AB201733)
  • WB

Lab

Western blot - HRP Anti-PDIA6 antibody [EPR10132(B)] (AB201733)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201733 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-PDIA6 antibody [EPR10132(B)] (ab201733) at 1/5000 dilution

Lane 1:

Kidney (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 48 kDa

Observed band size: 48 kDa,50 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10132(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDIA6 also known as Protein Disulfide Isomerase Family A Member 6 is a protein with a molecular mass of approximately 48 kDa. This protein resides primarily in the endoplasmic reticulum (ER) where it engages in the formation and rearrangement of disulfide bonds during the protein folding process. PDIA6 also facilitates chaperone activity aiding the proper folding of nascent proteins. Its expression is widespread but shows higher levels in secretory tissues reflecting its involvement in protein synthesis and processing.
Biological function summary

PDIA6 plays a critical role in maintaining cellular homeostasis by ensuring correct protein folding within the ER. It interacts with various substrates and is part of a larger protein complex along with other ER-resident chaperones and foldases. This protein also responds to ER stress by assisting in the unfolded protein response (UPR) a signaling cascade that restores normal function of the ER or triggers apoptosis if the stress is unresolved.

Pathways

PDIA6 holds a significant position in the folding modification and degradation of proteins via the ER-associated degradation (ERAD) pathway. This pathway involves collaboration with proteins such as BiP and ERp72 which together prevent accumulation of misfolded proteins. PDIA6 also contributes to the cellular redox homeostasis playing an important role in maintaining the balance of oxidative states within the cell which can influence pathways like the UPR.

PDIA6 associates with conditions characterized by ER stress including neurodegenerative disorders like Alzheimer's disease as well as cancer. In Alzheimer’s disease alterations in its function may affect the handling of misfolded proteins aligning it with amyloid precursor protein processing. In cancer its modulation of protein folding and stress responses links it to oncogenic pathways interacting with proteins such as p53 which can influence cell apoptosis and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed : 12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed : 24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed : 24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed : 15466936).
See full target information PDIA6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:485-492 PubMed39512105

2024

Impact of 6-OHDA injection and microtrauma in the rat on local brain amyloid beta protein concentrations in the affected area.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua A Roldán-Kalil,Sara E Vendrell-Gonzalez,Natalia Espinosa-Ponce,Jadier Colón-Vasques,Jescelica Ortiz-Rivera,Vassiliy Tsytsarev,Janaina M Alves,Mikhail Inyushin
View all publications
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Product promise

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