HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154]

• WB

Lab

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB197042)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197042 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042) at 1/3000 dilution

Lane 1:

HEK-293 (Human) Whole Cell Lysate (ab52256) at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 3:

LNCaP (Human prostate adenocarcinoma cell line) Whole Cell Lysate - clone FGC at 10 µg

Lane 4:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 22 kDa

Observed band size: 22 kDa

true

Exposure time: 4min

• WB

Lab

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB197042)

ab197042 was shown to specifically react with PRDX2 in wild-type HAP1 cells as signal was lost in PRDX2 (Peroxiredoxin 2) knockout cells. Wild-type and PRDX2 (Peroxiredoxin 2) knockout samples were subjected to SDS-PAGE. ab197042 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/3000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042) at 1/3000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PRDX2 (Peroxiredoxin 2) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 22 kDa

false

Exposure time: 20min