Mouse Monoclonal PGP9.5 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (AB197735), expandable thumbnail](https://content.abcam.com/products/images/hrp-pgp95-antibody-13c4-i3c4-neuronal-marker-ab197735--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7465.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (AB197735)")
![Western blot - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (AB197735), expandable thumbnail](https://content.abcam.com/products/images/hrp-pgp95-antibody-13c4-i3c4-neuronal-marker-ab197735--western-blot-img78507.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (AB197735)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Dog | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Sheep | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Guinea pig, Dog, Pig, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Species Rat | Dilution info 1/5000 | Notes - |
Species Human | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Guinea pig, Dog, Pig, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed:23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed:22212137, PubMed:25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed:12408865, PubMed:8639624, PubMed:9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed:22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed:25615526).
Alternative names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1
Recommended products
Mouse Monoclonal PGP9.5 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 13C4 / I3C4
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.
- Biological function summary
PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.
- Pathways
PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.
- Associated diseases and disorders
Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (ab197735), expandable thumbnail](https://content.abcam.com/products/images/hrp-pgp95-antibody-13c4-i3c4-neuronal-marker-ab197735--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7465.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (ab197735)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (ab197735)
PGP9.5 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-PGP9.5 antibody
IHC image of PGP9.5 staining in a section of formalin-fixed paraffin-embedded normal human pancreas*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197735, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![Western blot - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (ab197735), expandable thumbnail](https://content.abcam.com/products/images/hrp-pgp95-antibody-13c4-i3c4-neuronal-marker-ab197735--western-blot-img78507.jpg "Western blot - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (ab197735)")
Western blot - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (ab197735)
PGP9.5 Western blot staining using mouse Anti-PGP9.5 antibody
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197735 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406
All lanes: Western blot - HRP Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker (ab197735) at 1/5000 dilution
Lane 1: Brain (Human) Tissue Lysate - adult normal tissue at 20 µg
Lane 2: Brain (Rat) Tissue Lysate at 20 µg
Lane 3: Brain (Mouse) Tissue Lysate at 20 µg
Lane 4: Brain Cortex (Rat) Tissue Lysate at 20 µg
Lane 5: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 20 µg
Lane 6: Spinal Cord (Human) Tissue Lysate - adult normal at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 25 kDa
Exposure time: 8s
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