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AB9334

HRP Anti-Phosphoserine antibody

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(15 Publications)

Rabbit Polyclonal Phosphoserine antibody - conjugated to HRP. Suitable for ELISA, WB and reacts with Modified Amino Acid samples. Cited in 15 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphoserine.

View Alternative Names

PS, pSER, phospho-Ser

1 Images
Western blot - HRP Anti-Phosphoserine antibody (AB9334)
  • WB

Unknown

Western blot - HRP Anti-Phosphoserine antibody (AB9334)

Western blotting of mouse brain extract with ab9334 at a 1/200 dilution on a 12% SDS gel. 50 µg protein loaded in each lane. Antibody incubated for 2 hours incubation at room temperature.

All lanes:

Western blot - HRP Anti-Phosphoserine antibody (ab9334)

true

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

ELISA, WB

applications

Specificity

Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Immunoaffinity chromatography with phosphoserine-agarose.
Storage buffer
pH: 6 - 8 Constituents: Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoserine often referred to by its common alternate name "P-Ser" represents a phosphorylated form of the amino acid serine. This small molecule under a molecular mass of near 166 Daltons plays an important role in cellular signaling. It is expressed widely across various cell types particularly in tissues with high cell turnover. Phosphoserine results from the action of kinases which transfer a phosphate group from ATP to the serine's hydroxyl group. This phosphorylation process provides a critical modulatory mechanism that impacts protein function and cellular pathways.
Biological function summary

Phosphorylated serine residues are involved in controlling a multitude of cellular processes including cell cycle regulation and apoptosis. These phosphorylated regions form parts of protein kinases phosphatases and glycoproteins acting as switches for cellular signaling. Phosphoserine is often part of multi-protein complexes binding to other proteins and influencing their activity through changes in conformation. This active participation in biological pathways signifies its significant influence in maintaining cellular homeostasis.

Pathways

Phosphoserine plays a significant role in the MAPK signaling and PI3K/AKT pathways both important for cell growth and survival. It interacts with proteins like AKT and MAPK facilitating cellular responses to extracellular stimuli. This phosphorylation state dictates the activation or inactivation of these proteins effectively regulating key steps in signal transduction and transcriptional control. Such pathways highlight the importance of phosphoserine in coordinating complex cellular activities.

Aberrations in phosphoserine activity have been linked to conditions such as cancer and neurodegenerative diseases. In cancer dysregulation in its phosphorylation status may lead to uncontrolled cellular proliferation and resistance to apoptosis. Proteins such as p53 which plays a pivotal role in DNA repair and cell cycle arrest often show altered phosphorylation patterns in the presence of disease. Similarly in neurodegenerative disorders improper phosphorylation of tau proteins is a hallmark of conditions like Alzheimer's disease pointing to the critical involvement of phosphoserine and its associated pathways in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Phosphoserine
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:820-835 PubMed40164688

2025

MSK1 promotes colorectal cancer metastasis by increasing Snail protein stability through USP5-mediated Snail deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Keun-Seok Hong,Ki-Jun Ryu,Hyemin Kim,Minju Kim,Seung-Ho Park,Taeyoung Kim,Jung Wook Yang,Cheol Hwangbo,Kwang Dong Kim,Young-Jun Park,Jiyun Yoo

The Journal of biological chemistry 300:107542 PubMed38992436

2024

Activation of nemo-like kinase in diamond blackfan anemia suppresses early erythropoiesis by preventing mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mark C Wilkes,Aya Shibuya,Y Lucy Liu,Kailen Mark,Jaqueline Mercado,Mallika Saxena,Ryan S Sathianathen,Hye Na Kim,Bertil Glader,Paraic Kenny,Kathleen M Sakamoto

International journal of biological sciences 18:3237-3250 PubMed35637969

2022

Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Liu,Zhenzhen Chen,Shuangyue Li,Ling Jin,Xiao Cui,Changting Cui,Yue Deng,Qiannan Gao,Luyun Fan,Yaping Niu,Wenjie Wang,Chunmei Cui,Jiuchang Zhong,Qinghua Cui,Bin Geng,Jun Cai

Plant physiology 187:917-930 PubMed34608955

2021

Plant casein kinases phosphorylate and destabilize a cyclin-dependent kinase inhibitor to promote cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Li Qu,Zhuang Wei,Hu-Hui Chen,Tao Liu,Kan Liao,Hong-Wei Xue

Nature communications 11:3344 PubMed32620751

2020

Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase.

Applications

Unspecified application

Species

Unspecified reactive species

M C Wilkes,K Siva,J Chen,G Varetti,M Y Youn,H Chae,F Ek,R Olsson,T Lundbäck,D P Dever,T Nishimura,A Narla,B Glader,H Nakauchi,M H Porteus,C E Repellin,H T Gazda,S Lin,M Serrano,J Flygare,K M Sakamoto

Journal of leukocyte biology 89:63-73 PubMed20940326

2010

Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction.

Applications

WB

Species

Unspecified reactive species

Mario Martínez-Florensa,Antonio García-Blesa,José Yélamos,Alba Muñoz-Suano,Margarita Domínguez-Villar,Rut Valdor,Antonio Alonso,Francisco García-Cózar,Pedro Aparicio,Bernard Malissen,Enrique Aguado

Cellular signalling 21:34-42 PubMed18940248

2008

Identification of PP2A as a novel interactor and regulator of TRIP-Br1.

Applications

WB

Species

Unspecified reactive species

Zhi Jiang Zang,Lakshman Gunaratnam,Jit Kong Cheong,Li Yun Lai,Li-Li Hsiao,Eileen O'Leary,Xiaoming Sun,Manuel Salto-Tellez,Joseph V Bonventre,Stephen I-Hong Hsu

PloS one 3:e3430 PubMed18927610

2008

Signaling flux redistribution at toll-like receptor pathway junctions.

Applications

WB

Species

Unspecified reactive species

Kumar Selvarajoo,Yasunari Takada,Jin Gohda,Mohamed Helmy,Shizuo Akira,Masaru Tomita,Masa Tsuchiya,Jun-Ichiro Inoue,Koichi Matsuo

Journal of immunology (Baltimore, Md. : 1950) 180:8192-203 PubMed18523285

2008

Platelet-activating factor-mediated endosome formation causes membrane translocation of p67phox and p40phox that requires recruitment and activation of p38 MAPK, Rab5a, and phosphatidylinositol 3-kinase in human neutrophils.

Applications

WB

Species

Unspecified reactive species

Nathan J D McLaughlin,Anirban Banerjee,Samina Y Khan,Janet L Lieber,Marguerite R Kelher,Fabia Gamboni-Robertson,Forest R Sheppard,Ernest E Moore,Gary W Mierau,David J Elzi,Christopher C Silliman

FEBS letters 582:365-71 PubMed18082140

2007

Involvement of MAPK signaling molecules and Runx2 in the NELL1-induced osteoblastic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuyuki Bokui,Takayuki Otani,Koichi Igarashi,Junichiro Kaku,Mitsuo Oda,Tadahiro Nagaoka,Masaharu Seno,Kenji Tatematsu,Toshihide Okajima,Takashi Matsuzaki,Kang Ting,Katsuyuki Tanizawa,Shun'ichi Kuroda
View all publications
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