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Rabbit Polyclonal Phosphotyrosine antibody - conjugated to HRP. Suitable for IP, ELISA, WB and reacts with Modified Amino Acid samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.


Images

Western blot - HRP Anti-Phosphotyrosine antibody (AB9329), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

HRP

Storage buffer

pH: 6 - 8
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Chemical / Small Molecule corresponding to Phosphotyrosine.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPELISAWB
Modified Amino Acid
Expected
Expected
Tested

Expected
Expected

Species

Modified Amino Acid

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Modified Amino Acid

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Modified Amino Acid

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Alternative names

Recommended products

Rabbit Polyclonal Phosphotyrosine antibody - conjugated to HRP. Suitable for IP, ELISA, WB and reacts with Modified Amino Acid samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.

Alternative names

Key facts

Isotype

IgG

Conjugation

HRP

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Chemical / Small Molecule corresponding to Phosphotyrosine.
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

Immunoaffinity chromatography with phosphotyrosine-agarose.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

Associated diseases and disorders

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

Product promise

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1 product image

  • Western blot - HRP Anti-Phosphotyrosine antibody (ab9329), expandable thumbnail

    Western blot - HRP Anti-Phosphotyrosine antibody (ab9329)

    Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B) Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

    Lane 1: Western blot - Biotin Anti-Phosphotyrosine antibody (Biotin Anti-Phosphotyrosine antibody ab9331)

    Lane 1: Western blot - HRP Anti-Phosphotyrosine antibody (ab9329)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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