AB202640

HRP Anti-Polyethylene glycol antibody [PEG-B-47]

RabMAb
Recombinant
What is this?

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(1 Publication)

Anti-Polyethylene glycol antibody [PEG-B-47] - HRP conjugated (ab202640) is a rabbit recombinant monoclonal antibody detecting Polyethylene glycol in Western Blot, ELISA.

- Biophysical QC for unrivalled batch-batch consistency

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3 Images

ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (AB202640)

ELISA

Lab

ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (AB202640)

Sandwich ELISA antigen dose-response curve using ab202640.
Primary antibody concentration - ab202640 (500ng/ml and 250ng/ml).
Antigen - MIgG-PEG 5K (0-1000ng/ml).
Coated antibody - Goat anti-Mouse IgG (1000ng/ml).

ELISA

Lab

ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (AB202640)

Direct ELISA antibody dose-response curve using ab202640. Primary antibody concentration range 0-1000ng/ml, antigen (MIgG-PEG 5K) concentration range 0-500ng/ml.

Lab

Western blot - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (AB202640)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640) at 1/200 dilution

All lanes:

BSA-PEG at 15 µg

true

Exposure time: 3min

Carrier free

Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free
Unconjugated

Anti-Polyethylene glycol antibody [PEG-B-47]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

PEG-B-47

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>Do Not Use TBS As Buffer</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), ELISA. Related products
Antibody clone PEG-B-47 is also available pre-conjugated to a variety of labels for your convenience – Anti-Polyethylene glycol HRP [PEG-B-47] (ab202640).

Other related products
We have a range of other formats of antibody clone [PEG-B-47] also available for your convenience: ab51257, Carrier free - ab170969, HRP - ab202640

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Polyethylene glycol

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology 36:758-764 PubMed30010674

2018

Reversal of indoleamine 2,3-dioxygenase-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Todd A Triplett,Kendra C Garrison,Nicholas Marshall,Moses Donkor,John Blazeck,Candice Lamb,Ahlam Qerqez,Joseph D Dekker,Yuri Tanno,Wei-Cheng Lu,Christos S Karamitros,Kyle Ford,Bing Tan,Xiaoyan M Zhang,Karen McGovern,Silvia Coma,Yoichi Kumada,Mena S Yamany,Enrique Sentandreu,George Fromm,Stefano Tiziani,Taylor H Schreiber,Mark Manfredi,Lauren I R Ehrlich,Everett Stone,George Georgiou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Polyethylene glycol antibody [PEG-B-47]

ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (AB202640)

ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (AB202640)

Western blot - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (AB202640)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise