Rabbit Recombinant Monoclonal PPP1A/PPP1CA antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Images
![Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB211372), expandable thumbnail](https://content.abcam.com/products/images/hrp-ppp1ca-ppp1cb-antibody-ep1511y-ab211372--western-blot-img67216.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB211372)")
Publications
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- PLoS pathogens 16:e10086692020Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhanying Hu et. al.PubMed 32702076
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Species Rat | Dilution info 1/5000 | Notes - |
Species Human | Dilution info 1/5000 | Notes - |
Associated Products
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1 product for Alternative Version
Target data
Function
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323). Together with PPP1CC (PP1-gamma subunit), dephosphorylates IFIH1/MDA5 and RIG-I leading to their activation and a functional innate immune response (PubMed:23499489). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed:35768504, PubMed:36175670). (Microbial infection) Necessary for alphaviruses replication.
Additional Targets
PPP1CB
Alternative names
PPP1A, PPP1CA, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A
Recommended products
Rabbit Recombinant Monoclonal PPP1A/PPP1CA antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1511Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein targets PPP1CA and PPP1CB also known as protein phosphatase 1 catalytic subunits alpha and beta function as serine/threonine phosphatases. They both play roles in a range of cellular processes. The molecular weight of PPP1CA is approximately 37 kDa while PPP1CB is around 38 kDa. These phosphatases express widely across various tissues including the brain muscle and liver contributing to their involvement in diverse cellular functions. Their distribution suggests a broad range of activities across different physiological systems.
- Biological function summary
Protein phosphatase 1 catalytic subunits like PPP1CA and PPP1CB regulate important processes such as cell division and glycogen metabolism. These subunits typically are part of larger multiprotein complexes allowing for specific substrate targeting. By dephosphorylating specific proteins they act as regulatory signals within cellular systems influencing numerous biochemical pathways and responses. In neuronal tissues for example these phosphatases impact learning and memory by regulating synaptic plasticity through dephosphorylation cycles.
- Pathways
These phosphatase subunits engage in the MAP kinase and PI3K-Akt signaling pathways. In the MAP kinase pathway they interact with proteins such as ERK helping regulate cell proliferation and differentiation. In the PI3K-Akt pathway they modulate the activity of key components influencing processes like cell survival and growth. The regulatory function of PPP1CA and PPP1CB within these pathways facilitates critical cellular responses to various extracellular signals thereby affecting the overall cellular physiology.
- Associated diseases and disorders
Alterations in PPP1CA and PPP1CB activity have links to several health concerns including cancer and diabetes. In certain cancers abnormal expression or mutation of these phosphatases affects cell cycle control leading to unchecked cell growth. In diabetes PPP1CA and PPP1CB influence glycogen metabolism where their dysregulation contributes to impaired glucose homeostasis. These targets connect to other proteins like GSK-3 in cancer and AKT1 in diabetes highlighting their diverse interactions and potential as targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372), expandable thumbnail](https://content.abcam.com/products/images/hrp-ppp1ca-ppp1cb-antibody-ep1511y-ab211372--western-blot-img67216.jpg "Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)")
Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372) at 1/5000 dilution
Lane 1: Brain (Mouse) Tissue Lysate at 10 µg
Lane 2: Brain (Rat) Tissue Lysate at 10 µg
Lane 3: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 36 kDa
Exposure time: 3min
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