JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB306213

HRP Anti-PPP1R1A antibody [EP902Y]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PPP1R1A antibody - conjugated to HRP.

View Alternative Names

IPP1, PPP1R1A, Protein phosphatase 1 regulatory subunit 1A, Protein phosphatase inhibitor 1, I-1, IPP-1

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP902Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 1 regulatory inhibitor subunit 1A also known as PPP1R1A or inhibitor-1 (I-1) plays a significant role in cellular regulation as a phosphatase inhibitor. Mechanically it regulates the activity of protein phosphatase 1 (PP1) an essential enzyme in various cellular processes. Its molecular weight is approximately 19 kDa. PPP1R1A is expressed in many tissues showing notable levels in cardiac and skeletal muscle where it helps modulate responses to various stimuli.
Biological function summary

PPP1R1A modulates signaling pathways by influencing the dephosphorylation of substrates which is a critical step in signal transduction. It is not typically part of a complex but interacts closely with protein phosphatase 1. This interaction allows for the effective regulation of phosphorylation states within cells impacting cell cycle control muscle contraction glycogen metabolism and more. Its regulatory capabilities are important for maintaining cellular homeostasis.

Pathways

The regulatory activities of PPP1R1A significantly impact the cAMP signaling pathway and the regulation of glycogen metabolism. Within the cAMP pathway PPP1R1A regulates the activity of PP1 influencing processes like muscle contraction and gene expression. It interacts with proteins such as PKA which phosphorylates PPP1R1A activating its inhibitory function. This regulation affects downstream cellular activities by modulating phosphorylation levels of key substrates within these pathways.

Alterations in PPP1R1A expression or function have implications in cardiac diseases and diabetes. Increased or decreased activity of PPP1R1A can disrupt normal heart function due to its regulatory role in cardiac muscle contraction. Altered regulation within the cAMP and glycogen metabolism pathways can contribute to metabolic disorders like diabetes. Interactions with proteins such as PP1 and PKA are critical for these conditions highlighting PPP1R1A's role in disease progression and potential therapeutic targeting.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.
See full target information PPP1R1A
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB302716

Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)

  • 0
  • 0
Primary Antibodies

AB255610

Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (AB255610)

  • 5
  • 2
Primary Antibodies

AB305054

Anti-RIP3 antibody [EPR24374-135]

20ul selling size|RabMAb|Recombinant|Trial Size

Western blot - Anti-RIP3 antibody [EPR24374-135] (AB305054)

  • 0
  • 0
Primary Antibodies

AB312138

Alexa Fluor® 555 Anti-MUC13 antibody [EPR21901]

RabMAb|Recombinant

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com