AB203462

HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Prostaglandin E Receptor EP2/PTGER2 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

Prostaglandin E2 receptor EP2 subtype, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER2

1 Images

Lab

Western blot - HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB203462)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203462 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab203462) at 1/5000 dilution

Lane 1:

Placenta (Human) Tissue Lysate - adult normal tissue at 10 µg

Lane 2:

Lung (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

Lane 3:

JAR (Human Choriocarcinoma) Whole Cell Lysate at 10 µg

Lane 4:

WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 5:

Liver (Mouse) Tissue Lysate at 10 µg

Predicted band size: 40 kDa

Observed band size: 55 kDa

true

Exposure time: 10s

Carrier free

Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] - BSA and Azide free
Unconjugated

Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8030(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Human, Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E Receptor EP2 also known as PTGER2 or EP2 receptor is a G-protein coupled receptor with a molecular mass of approximately 53 kDa. This receptor primarily binds prostaglandin E2 (PGE2) mediating various cellular responses. EP2 receptor is expressed in tissues such as the brain kidneys lungs and immune cells. Its activation leads to the stimulation of adenylyl cyclase increasing levels of cyclic AMP (cAMP) within the cell.

Biological function summary

The EP2 receptor plays a role in vasodilation immune modulation and regulation of pain and inflammation. It does not act as part of a large protein complex but rather exerts its effects individually through its coupling with G-proteins. EP2 receptor stimulation results in smooth muscle relaxation and has implications in neuroinflammation and renal function.

Pathways

The EP2 receptor is involved in the prostaglandin signaling pathway and contributes to the inflammatory response pathway. It shares a connection with other receptors such as EP1 EP3 and EP4 which also bind PGE2 but induce different cellular responses. These pathways integrate signals that modulate processes like inflammation and immune responses highlighting EP2's role in maintaining physiological balance.

EP2 receptor activity is associated with conditions like asthma and Alzheimer's disease. In asthma the receptor's activity may influence airway hyperresponsiveness through its effects on smooth muscle and immune cells. In Alzheimer's EP2 receptor-related pathways may contribute to neuroinflammation which is linked with disease progression. Connections with proteins like COX-2 can be noted in these disorders as COX-2-dependent prostaglandin synthesis directly affects EP2 receptor signaling and subsequent cellular outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.

See full target information Prostaglandin E2 receptor EP2 subtype

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research communications 4:1369-1379 PubMed38709075

2024

Pan-Cancer Interrogation of B7-H3 (CD276) as an Actionable Therapeutic Target Across Human Malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Carly D Miller,John R Lozada,Nicholas A Zorko,Andrew Elliott,Allison Makovec,Milan Radovich,Elisabeth I Heath,Neeraj Agarwal,Rana R Mckay,Rohan Garje,Bruno R Bastos,Dave S B Hoon,Jacob J Orme,Oliver Sartor,Ari VanderWalde,Chadi Nabhan,George Sledge,Eugene Shenderov,Scott M Dehm,Emil Lou,Jeffrey S Miller,Justin H Hwang,Emmanuel S Antonarakis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

Western blot - HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB203462)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Biochemicals

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise