AB270144

HRP Anti-Rabbit IgG antibody [SP137]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(21 Publications)

Rabbit Recombinant Monoclonal IGHG antibody - conjugated to HRP. Suitable for IHC-P and reacts with Rabbit samples. Cited in 21 publications.

View Alternative Names

Ig gamma chain C region, Ig kappa-b9 chain C region, Ig kappa-b4 chain C region, K-BAS, Ig kappa-b4 chain C region, Ig lambda chain C region, Ig kappa-b5 chain C region

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (AB270144)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (AB270144)

IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND^®.

The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (AB270144)

IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal rat spleen*, performed on a Leica BOND^®.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (AB270144)

IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal mouse spleen*, performed on a Leica BOND^®.

The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Carrier free

Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free)
Unconjugated

Rabbit IgG, monoclonal [SP137] - isotype control
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Rabbit IgG antibody [SP137]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP137

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Rabbit

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rabbit": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG Fc

Additional targets

,KAC4_RABIT,,,

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40507985

2025

Potential Role of SESN3 in Linking Heart Failure with Preserved Ejection Fraction and Chronic Obstructive Pulmonary Disease via Autophagy Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Rongxin Zhu,Binhua Yuan,Yunlin Li,Xiangning Liu,Mingyue Huang,Boyang Jiao,Ying Sun,Sheng Gao,Xiaoqian Sun,Tianhua Liu,Yan Wu,Chun Li

Frontiers in microbiology 16:1570659 PubMed40207157

2025

protein of adhesion inhibits human urethral epithelial cells apoptosis via CypA/PI3K/AKT/mTOR-dependent autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Dan Luo,Hua Xiao,Zhuo Zeng,Haodang Luo,Siqi Gao,Xiaoqian Tang,Zhijia Huang,Yanhua Zeng

Chemical biology & drug design 105:e70096 PubMed40159917

2025

Breviscapine Attenuates Lipopolysaccharide-Induced Airway Dysfunction in Normal Human Bronchial Epithelial Cells by Suppressing the TLR4/MyD88 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaocong Zhao,Wanwan Li,Yanfeng Zhao,Xiaomin Sun

Molecular medicine (Cambridge, Mass.) 31:27 PubMed39871124

2025

Formononetin ameliorates polycystic ovary syndrome through suppressing NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Liu,Rui-Han Wang,Ke-Hua Wang

iScience 27:110467 PubMed39104411

2024

PIK3CA mutations enhance the adipogenesis of ADSCs in facial infiltrating lipomatosis through TRPV1.

Applications

Unspecified application

Species

Unspecified reactive species

Hongrui Chen,Bin Sun,Wei Gao,Yajing Qiu,Wei Wei,Yongguo Li,Wei Ye,Haoliang Song,Chen Hua,Xiaoxi Lin

Biochemical genetics 63:1620-1634 PubMed38600398

2024

C1q/Tumor Necrosis Factor-Related Protein-9 Enhances Macrophage Cholesterol Efflux and Improves Reverse Cholesterol Transport via AMPK Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaosu Song,Gaizhen Liu,Yunfei Bin,Rui Bai,Bin Liang,Huiyu Yang

Acta biochimica et biophysica Sinica 56:607-620 PubMed38414350

2024

miR-29b-1-5p exacerbates myocardial injury induced by sepsis in a mouse model by targeting TERF2.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqing Jiang,Junmei Xu,Hua Zeng,Zhaojing Lin,Qiong Yi,Jiali Guo,Feng Xiao

Cell cycle (Georgetown, Tex.) 23:188-204 PubMed38357935

2024

ZC3H12A inhibits tumor growth and metastasis of breast cancer under hypoxic condition via the inactivation of IL-17 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongbing Luo,Fulan Yang,Kang Liu,Zhenluo Ding

Cardiology research 14:453-463 PubMed38187515

2024

Inhibition of the STIM1/Orai1 Signaling Pathway by Glycine Betaine Mitigates Myocardial Hypertrophy in Spontaneous Hypertension Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Xiao,Yan Zhao,Ke Ke Wang,Xiu Chun Yang,Hai Juan Hu,Yue Li,Yun Fei Xu,Zhen Tian Zhang,Shuai Wang,Jing Chao Lu

mBio 14:e0198723 PubMed37754570

2023

Butyrate protects against MRSA pneumonia via regulating gut-lung microbiota and alveolar macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Haoming Sun,Yiwei Chen,Qiang Niu,Yiting Dong,Mei Li,Ye Yuan,Xiaojun Yang,Qingzhu Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Rabbit IgG antibody [SP137]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (AB270144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (AB270144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (AB270144)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (21)

Product promise