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AB34767

HRP Anti-RFP antibody

4

(3 Reviews)

|

(8 Publications)

Rabbit Polyclonal RFP antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 8 publications. Immunogen corresponding to Recombinant Protein within Sea anenome Red fluorescent protein drFP583.

View Alternative Names

Red fluorescent protein drFP583, DsRed

1 Images
Western blot - HRP Anti-RFP antibody (AB34767)
  • WB

Supplier Data

Western blot - HRP Anti-RFP antibody (AB34767)

Lanes 1 - 2:

Western blot - HRP Anti-RFP antibody (ab34767) at 1/2500 dilution

Lanes 1 - 2:

Western blot - FITC Anti-RFP antibody (<a href='/en-us/products/primary-antibodies/fitc-rfp-antibody-ab34764'>ab34764</a>) at 1/2500 dilution

Lane 1:

HeLa cell extract at 10 µg

Lane 2:

HeLa lysate at 10 µg

Secondary

All lanes:

Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Protein within Sea anenome Red fluorescent protein drFP583. The exact immunogen used to generate this antibody is proprietary information.

Q9U6Y8

Specificity

Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Purification technique
Affinity purification
Purification notes
immunoaffinity chromatography was followed by solid phase adsorption to remove any unwanted reactivities.
Storage buffer
pH: 7.2 Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
See full target information Red fluorescent protein drFP583
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:4385 PubMed35902598

2022

Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo Enrique Gomez,Clément Chambaud,Josselin Lupette,Julie Castets,Stéphanie Pascal,Lysiane Brocard,Lise Noack,Yvon Jaillais,Jérôme Joubès,Amélie Bernard

Applied microbiology and biotechnology 104:10503-10513 PubMed33141296

2020

Identification of a novel cell-penetrating peptide derived from the capsid protein of chicken anemia virus and its application in gene delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Gaowei Hu,Yingjie Miao,Xi Luo,Wenhui Chu,Yongqian Fu

Nature 572:270-274 PubMed31291642

2019

Mechanisms of RALF peptide perception by a heterotypic receptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xiao,Martin Stegmann,Zhifu Han,Thomas A DeFalco,Katarzyna Parys,Li Xu,Youssef Belkhadir,Cyril Zipfel,Jijie Chai

Science signaling 12: PubMed31015291

2019

Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Robert F Hennigan,Jonathan S Fletcher,Steven Guard,Nancy Ratner

PloS one 9:e103839 PubMed25075518

2014

Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.

Applications

WB

Species

Unspecified reactive species

Jessica Thevenard,Laurie Verzeaux,Jerôme Devy,Nicolas Etique,Albin Jeanne,Christophe Schneider,Cathy Hachet,Géraldine Ferracci,Marion David,Laurent Martiny,Emmanuelle Charpentier,Michel Khrestchatisky,Santiago Rivera,Stéphane Dedieu,Hervé Emonard

PLoS biology 11:e1001732 PubMed24339748

2013

A downy mildew effector attenuates salicylic acid-triggered immunity in Arabidopsis by interacting with the host mediator complex.

Applications

WB

Species

Unspecified reactive species

Marie-Cécile Caillaud,Shuta Asai,Ghanasyam Rallapalli,Sophie Piquerez,Georgina Fabro,Jonathan D G Jones

eLife 2:e00444 PubMed23580231

2013

SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Wei Chen,He Wang,Kanika Bajaj,Pengcheng Zhang,Zhuo-Xian Meng,Danjun Ma,Yongsheng Bai,Hui-Hui Liu,Elizabeth Adams,Andrea Baines,Genggeng Yu,Maureen A Sartor,Bin Zhang,Zhengping Yi,Jiandie Lin,Stephen G Young,Randy Schekman,David Ginsburg

PloS one 5:e9008 PubMed20126408

2010

Visualisation of PCNA monoubiquitination in vivo by single pass spectral imaging FRET microscopy.

Applications

WB

Species

Unspecified reactive species

Christopher Batters,Hannah Zhu,Julian E Sale
View all publications
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