Rabbit Recombinant Monoclonal RFP antibody - conjugated to HRP. Suitable for WB and reacts with Transfected cell lysate - Entacmaea quadricolor samples.
Images
![Western blot - HRP Anti-RFP antibody [EPR18992] (AB324002), expandable thumbnail](https://content.abcam.com/products/images/hrp-rfp-antibody-epr18992-ab324002--western-blot-img446830.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-RFP antibody [EPR18992] (AB324002)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Transfected cell lysate - Entacmaea quadricolor | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Entacmaea quadricolor | Dilution info 1/1000 | Notes - |
Alternative names
Red fluorescent protein eqFP611, GFP-like chromoprotein
Rabbit Recombinant Monoclonal RFP antibody - conjugated to HRP. Suitable for WB and reacts with Transfected cell lysate - Entacmaea quadricolor samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18992
- Purification technique
- Affinity purification Protein A
- Specificity
We haven't test this antibody with DsRed.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - HRP Anti-RFP antibody [EPR18992] (ab324002), expandable thumbnail](https://content.abcam.com/products/images/hrp-rfp-antibody-epr18992-ab324002--western-blot-img446830.jpg "Western blot - HRP Anti-RFP antibody [EPR18992] (ab324002)")
Western blot - HRP Anti-RFP antibody [EPR18992] (ab324002)
RFP Western blot staining using rabbit Anti-RFP antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the higher MW band at approximately 50 kDa is unknown.
Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) (1:200000) (36KDa).
Total protein control: Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) (1:5000)(30 kDa).
All lanes: Western blot - HRP Anti-RFP antibody [EPR18992] (ab324002) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-his-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with Entacmaea quadricolor RFP containing a myc-his-tag, whole cell lysate at 20 µg
Observed band size: 30 kDa, 36 kDa
Exposure time: 10s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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