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Rabbit Recombinant Monoclonal RPE65 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (AB324030), expandable thumbnail
  • Western blot - HRP Anti-RPE65 antibody [EPR22579-44] (AB324030), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (AB324030), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (AB324030), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (AB324030), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/400
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/400
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:16116091). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed:17848510). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid (PubMed:28874556). The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).

Additional Targets

Rpe65, Rpe65

Alternative names

Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase, RPE65

Rabbit Recombinant Monoclonal RPE65 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22579-44
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030)

    RPE65 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human retina using rabbit Anti-RPE65 antibody

    Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling RPE65 with ab324030 at 1/400 (1.25 ug/ml) dilution.

    Cytoplasmic staining on pigment epithelium cells of human retina.

    The section was incubated with ab324030 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Western blot - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030), expandable thumbnail

    Western blot - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030)

    RPE65 Western blot staining using rabbit Anti-RPE65 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds, Lanes 2-3: 15 seconds.

    All lanes: Western blot - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030) at 1/1000 dilution

    Lane 1: Human eyeball tissue lysate at 20 µg

    Lane 2: Mouse eyeball tissue lysate at 20 µg

    Lane 3: Rat eyeball tissue lysate at 20 µg

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030)

    RPE65 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human skeletal muscle tissue using rabbit Anti-RPE65 antibody

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling RPE65 with ab324030 at 1/400 (1.25 ug/ml) dilution.

    Negative control: no staining on human skeletal muscle.

    The section was incubated with ab324030 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030)

    RPE65 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat retina using rabbit Anti-RPE65 antibody

    Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling RPE65 with ab324030 at 1/400 (1.25 ug/ml) dilution.

    Cytoplasmic staining on pigment epithelium cells of rat retina.

    The section was incubated with ab324030 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RPE65 antibody [EPR22579-44] (ab324030)

    RPE65 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse retina using rabbit Anti-RPE65 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling RPE65 with ab324030 at 1/400 (1.25 ug/ml) dilution.

    Cytoplasmic staining on pigment epithelium cells of mouse retina.

    The section was incubated with ab324030 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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