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Rabbit Recombinant Monoclonal Scavenging Receptor SR-BI antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

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Images

Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB206233), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB206233), expandable thumbnail
  • Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB206233), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

2 products for Alternative Version

Target data

Function

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells (PubMed:12016218, PubMed:12519372, PubMed:21226579). Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux (PubMed:26965621). Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (PubMed:12016218). (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry (PubMed:12356718, PubMed:12913001, PubMed:18000990). Binding between SCARB1 and the hepatitis C virus glycoprotein E2 is independent of the genotype of the viral isolate (PubMed:12356718). (Microbial infection) Mediates uptake of M.fortuitum, E.coli and S.aureus. (Microbial infection) Facilitates the entry of human coronavirus SARS-CoV-2 by acting as an entry cofactor through HDL binding.

Alternative names

CD36, CD36L1, CLA1, SCARB1, Scavenger receptor class B member 1, SRB1, CD36 and LIMPII analogous 1, CD36 antigen-like 1, SR-BI, CLA-1

Recommended products

Rabbit Recombinant Monoclonal Scavenging Receptor SR-BI antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1556Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Scavenging Receptor Class B Type 1 (SR-BI) also known as CLA-1 in humans is a membrane protein involved in the selective uptake of lipids. This protein has a molecular mass of approximately 82 kDa and is primarily expressed in the liver and non-placental tissues such as adrenal glands and ovaries. SR-BI mediates the transfer of cholesterol esters from high-density lipoprotein (HDL) to cells while playing a role in reverse cholesterol transport. Its function is essential for maintaining cholesterol homeostasis.

Biological function summary

SR-BI contributes to lipid metabolism by influencing the dynamics of cholesterol and lipid circulation within the body. It forms part of the larger HDL receptor complex which facilitates the selective uptake of lipids without internalizing the entire lipoprotein. Through this mechanism SR-BI assists cells in acquiring necessary components for membrane synthesis and hormone production.

Pathways

SR-BI integrates into the cholesterol biosynthesis pathway and the reverse cholesterol transport pathway. In these pathways SR-BI works closely with proteins like ATP-binding cassette transporter A1 (ABCA1) and lecithin-cholesterol acyltransferase (LCAT). These interactions promote the efflux and transport of cholesterol therefore playing a vital role in lipid regulation and metabolic processes.

Associated diseases and disorders

SR-BI's malfunction can lead to cardiovascular diseases due to its role in cholesterol metabolism. Elevated levels of HDL cholesterol without functional SR-BI impair reverse cholesterol transport potentially leading to atherosclerosis. Moreover SR-BI interacts with apolipoprotein A-I (apoA-I) a major protein component of HDL impacting its efficacy and connection to coronary artery disease. Understanding SR-BI pathways and their interactions can provide insights into therapeutic strategies for treating cholesterol-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233), expandable thumbnail

    Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233)

    ab206233 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in SCARB1 (Scavenging Receptor SR-BI) knockout cells. Wild-type and SCARB1 (Scavenging Receptor SR-BI) knockout samples were subjected to SDS-PAGE. ab206233 and Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    All lanes: Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233) at 1/2000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: SCARB1 (Scavenging Receptor SR-BI) knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 60 kDa

    Observed band size: 76 kDa

    Exposure time: 20min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233)

    IHC image of Scavenging Receptor SR-BI staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab206233, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233), expandable thumbnail

    Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab206233 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233) at 1/2000 dilution

    All lanes: Liver (Mouse) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Observed band size: 76 kDa

    Exposure time: 8min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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