Rabbit Recombinant Monoclonal Serum Amyloid P/SAP antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--western-blot-img446052.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)")
![Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--western-blot-img446051.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446080.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446079.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/20000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
Alternative names
PTX2, APCS, Serum amyloid P-component, SAP, 9.5S alpha-1-glycoprotein
Rabbit Recombinant Monoclonal Serum Amyloid P/SAP antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1018Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.
- Biological function summary
SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.
- Pathways
SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.
- Associated diseases and disorders
SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--western-blot-img446052.jpg "Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)")
Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)
Serum Amyloid P/SAP Western blot staining of Human liver tissue lysate using rabbit Anti-Serum Amyloid P/SAP antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the higher MW band at approximately 60 kDa (in lane 1) is unknown.
All lanes: Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994) at 1/1000 dilution
All lanes: Human liver tissue lysate at 20 µg
Observed band size: 25 kDa
Exposure time: 1s
![Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--western-blot-img446051.jpg "Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)")
Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)
Serum Amyloid P/SAP Western blot staining of Human serum tissue lysate using rabbit Anti-Serum Amyloid P/SAP antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994) at 1/20000 dilution
All lanes: Human serum tissue lysate at 20 µg
Observed band size: 25 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446080.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)
Serum Amyloid P/SAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human endometrial carcinoma using rabbit Anti-Serum Amyloid P/SAP antibody
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Serum Amyloid P/SAP with ab323994 at 1/100 (5.0 ug/ml) dilution.
Positive staining on human endometrial carcinoma.
The section was incubated with ab323994 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994), expandable thumbnail](https://content.abcam.com/products/images/hrp-serum-amyloid-p-sap-antibody-ep1018y-ab323994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446079.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994)
Serum Amyloid P/SAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human testis using rabbit Anti-Serum Amyloid P/SAP antibody
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Serum Amyloid P/SAP with ab323994 at 1/100 (5.0 ug/ml) dilution.
Positive staining on human testis.
The section was incubated with ab323994 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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