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AB323994

HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y]

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Rabbit Recombinant Monoclonal Serum Amyloid P/SAP antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

PTX2, APCS, Serum amyloid P-component, SAP, 9.5S alpha-1-glycoprotein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Serum Amyloid P/SAP with ab323994 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human endometrial carcinoma.
The section was incubated with ab323994 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Serum Amyloid P/SAP with ab323994 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human testis.
The section was incubated with ab323994 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)
  • WB

Supplier Data

Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994) at 1/20000 dilution

All lanes:

Human serum tissue lysate at 20 µg

Observed band size: 25 kDa

false

Exposure time: 1s

Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)
  • WB

Supplier Data

Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (AB323994)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 60 kDa (in lane 1) is unknown.

All lanes:

Western blot - HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab323994) at 1/1000 dilution

All lanes:

Human liver tissue lysate at 20 µg

Observed band size: 25 kDa

false

Exposure time: 1s

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Serum Amyloid P/SAP antibody [EP1018Y]

  • Unconjugated

    Anti-Serum Amyloid P/SAP antibody [EP1018Y]

  • Carrier free

    Anti-Serum Amyloid P/SAP antibody [EP1018Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1018Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB246537

Human SAP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SAP ELISA Kit (AB246537)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.
Biological function summary

SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.

Pathways

SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.

SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
See full target information APCS
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com