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AB193891

HRP Anti-STAT1 alpha antibody [EPYR2154]

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(1 Publication)

Rabbit Recombinant Monoclonal STAT1 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

2 Images
Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (AB193891)
  • WB

Lab

Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (AB193891)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193891 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 4:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 91 kDa

true

Exposure time: 2min

Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (AB193891)
  • WB

Lab

Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (AB193891)

ab193891 was shown to specifically react with STAT1 alpha in wild-type HAP1 cells as signal was lost in STAT1 knockout cells. Wild-type and STAT1 knockout samples were subjected to SDS-PAGE. ab193891 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891) at 1/2000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

STAT1 knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 91 kDa

false

Exposure time: 2min

  • Carrier free

    Anti-STAT1 alpha antibody [EPYR2154] - BSA and Azide free

  • Unconjugated

    Anti-STAT1 alpha antibody [EPYR2154]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPYR2154

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Human, Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT1 alpha also known as Signal Transducer and Activator of Transcription 1-alpha is a protein with a mass of approximately 91 kDa. It operates in the cell nucleus and cytoplasm to mediate signal transduction and activation of transcription. STAT1 alpha does this by dimerizing following phosphorylation enabling its nuclear translocation. It is broadly expressed in many tissues including immune cells. This expression plays an important role in response to cytokines and other signaling molecules.
Biological function summary

STAT1 alpha functions as a critical component of the interferon signaling pathway. It is part of a transcription factor complex that regulates the expression of genes involved in cell proliferation differentiation and immune response. STAT1 alpha mediates its effects by forming homo- or heterodimers with related proteins and binding to specific DNA sequences. This interaction facilitates the transcription of genes necessary for innate and adaptive immunity highlighting its fundamental role in host defense mechanisms.

Pathways

STAT1 alpha participates in the JAK-STAT signaling pathway and interferon signaling pathways. It interacts with Janus kinases (JAKs) after cytokine stimulation resulting in its phosphorylation and dimerization. This pathway links it with other STAT family members such as STAT2 and STAT3 to regulate gene expression. These pathways ensure proper immune function and cell growth control indicating STAT1 alpha's essential role in various cellular processes.

STAT1 alpha is associated with autoimmune diseases and certain cancers. Its malfunction can result in inadequate immune responses or excessive inflammation. In some cancers altered STAT1 alpha activity contributes to unchecked cell proliferation. STAT1 alpha interacts with various proteins such as IFNGR1 and IRF1 in these disease states affecting their signaling pathways. Its dysregulation indicates a potential target for therapeutic intervention in these disorders making it of interest for medical research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed : 12764129, PubMed : 12855578, PubMed : 15322115, PubMed : 23940278, PubMed : 34508746, PubMed : 35568036, PubMed : 9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed : 28753426, PubMed : 35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed : 28753426, PubMed : 35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed : 26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed : 8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed : 15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed : 32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed : 32209697). Involved in food tolerance in small intestine : associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).
See full target information STAT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

APL bioengineering 5:026104 PubMed34027283

2021

Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Mathieu Danoy,Yannick Tauran,Stephane Poulain,Rachid Jellali,Johanna Bruce,Marjorie Leduc,Morgane Le Gall,Yuta Koui,Hiroshi Arakawa,Francoise Gilard,Bertrand Gakiere,Yukio Kato,Charles Plessy,Taketomo Kido,Atsushi Miyajima,Yasuyuki Sakai,Eric Leclerc
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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