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AB324294

HRP Anti-Strep-tag II antibody [EPR28119-43]

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Rabbit Recombinant Monoclonal Strep-tag II antibody - conjugated to HRP. Suitable for WB, I-ELISA and reacts with Tag samples.

View Alternative Names

Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II

2 Images
ELISA - HRP Anti-Strep-tag II antibody [EPR28119-43] (AB324294)
  • ELISA

Supplier Data

ELISA - HRP Anti-Strep-tag II antibody [EPR28119-43] (AB324294)

Indirect ELISA analysis of ab324294 at 1000-0 ng/ml.

Antigen : Strep-tag II.

Antigen concentration : 1000 ng/ml

Western blot - HRP Anti-Strep-tag II antibody [EPR28119-43] (AB324294)
  • WB

Supplier Data

Western blot - HRP Anti-Strep-tag II antibody [EPR28119-43] (AB324294)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - HRP Anti-Strep-tag II antibody [EPR28119-43] (ab324294) at 1/50000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Strep-tag II expression vector containing a His tag whole cell lysate at 20 µg

Observed band size: 30 kDa,36 kDa

false

Exposure time: 3s

  • Unconjugated

    Anti-Strep-tag II antibody [EPR28119-43]

  • Carrier free

    Anti-Strep-tag II antibody [EPR28119-43] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28119-43

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Strep-tag II
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com