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Rabbit Recombinant Monoclonal Synaptophysin antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB195520), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB195520), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Expected
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

-

Species
Rat
Dilution info
1/10000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

2 products for Alternative Product

Target data

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Alternative names

Synaptophysin, Major synaptic vesicle protein p38, SYP

Recommended products

Rabbit Recombinant Monoclonal Synaptophysin antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
YE269
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Associated diseases and disorders

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker (ab195520), expandable thumbnail

    Western blot - HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker (ab195520)

    Synaptophysin Western blot staining using rabbit Anti-Synaptophysin antibody

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195520 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker (ab195520) at 1/1000 dilution

    Lane 1: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: Brain (Rat) Tissue Lysate at 10 µg

    Lane 3: Brain (Mouse) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 37 kDa

    Exposure time: 2s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker (ab195520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker (ab195520)

    Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Synaptophysin antibody

    IHC image of Synaptophysin staining in a section of formalin-fixed paraffin-embedded normal human pancreas tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195520 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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