AB200788

HRP Anti-TRIM25/EFP antibody [EPR7315]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TRIM25/EFP antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EFP, RNF147, ZNF147, TRIM25, E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, RING-type E3 ubiquitin transferase, RING-type E3 ubiquitin transferase TRIM25, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147

1 Images

Lab

Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (AB200788)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200788 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788) at 1/5000 dilution

All lanes:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Predicted band size: 71 kDa

Observed band size: 71 kDa

true

Exposure time: 8min

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TRIM25/EFP antibody [EPR7315]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7315

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM25 also known as EFP is a protein involved in antiviral response mechanisms. TRIM25 has a molecular mass of approximately 70 kDa and is part of the TRIM (Tripartite Motif) family which contains three distinct zinc-binding domains followed by a coiled-coil region. This protein exhibits strong expression in a variety of tissues including lung liver and intestines. As a member of the E3 ubiquitin ligase enzyme family TRIM25 plays a direct role in protein ubiquitination which tags specific proteins for degradation by the proteasome.

Biological function summary

TRIM25 functions as a significant modulator of the innate immune response against viral infections. In cells it acts within the RIG-I signaling complex directly promoting the RIG-I-MAVS pathway that detects viral RNA. TRIM25 mediates the ubiquitination of RIG-I allowing for its proper activation and subsequent signal transduction facilitating a robust antiviral state. TRIM25's role extends beyond antiviral defenses to include regulatory functions in cell proliferation and differentiation.

Pathways

TRIM25 engages actively in the innate immune signaling and JAK-STAT pathways. It interacts and cooperates with RIG-I in the recognition and response to viral tRNA leading to the production of interferons that establish an antiviral environment. Furthermore TRIM25 also connects with proteins like TRIM21 which further engages the immune response. This interaction enhances the overall ability of cells to fend off viral pathogens efficiently.

Altered TRIM25 function is associated with conditions like systemic lupus erythematosus (SLE) and various cancers. In the context of SLE TRIM25's regulatory role in innate immunity can affect the severity and progression of this autoimmune disorder. Additionally in some cancers abnormal TRIM25 expression can influence cell cycle regulation and metastasis. Through its ubiquitination activities TRIM25 interacts directly with proteins such as p53 affecting tumor suppression pathways and contributing to the complexity of disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed : 16352599). Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1 (PubMed : 17392790, PubMed : 29357390, PubMed : 30193849, PubMed : 31710640, PubMed : 33849980, PubMed : 36045682). Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed : 17392790, PubMed : 23950712). Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed : 30193849). Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed : 16352599, PubMed : 17069755). Mediates estrogen action in various target organs (PubMed : 22452784). Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed : 22452784). Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity). Plays an essential role in the antiviral activity of ZAP/ZC3HAV1; an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA (PubMed : 28060952, PubMed : 28202764). Mediates also the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability (PubMed : 36067236).

See full target information TRIM25

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:1820 PubMed29739942

2018

Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Marios G Koliopoulos,Mathilde Lethier,Annemarthe G van der Veen,Kevin Haubrich,Janosch Hennig,Eva Kowalinski,Rebecca V Stevens,Stephen R Martin,Caetano Reis e Sousa,Stephen Cusack,Katrin Rittinger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-TRIM25/EFP antibody [EPR7315]

Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (AB200788)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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