JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB303030

HRP Anti-TROP2 antibody [EPR20043]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TROP2 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

GA733-1, M1S1, TROP2, TACSTD2, Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TROP2 antibody [EPR20043] (AB303030)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TROP2 antibody [EPR20043] (AB303030)

Negative staining of TROP2 in a section of formalin-fixed paraffin-embedded human normal cerebral cortex*.

Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. The section was then incubated with ab303030 at 5µg/mL, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TROP2 antibody [EPR20043] (AB303030)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TROP2 antibody [EPR20043] (AB303030)

IHC image of TROP2 staining in a section of formalin-fixed paraffin-embedded normal human breast*.

Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. The section was then incubated with ab303030 at 5µg/mL, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20043

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB322439

Human TROP2 ELISA Kit- Extracellular

Recombinant|SimpleStep

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

  • 0
  • 0
Primary Antibodies

AB199093

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

  • 0
  • 0
Primary Antibodies

AB193895

Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] (AB193895)

  • 5
  • 4
Primary Antibodies

AB192467

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

  • 5
  • 5
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TROP2 also known as TACSTD2 or tumor-associated calcium signal transducer 2 is a single-pass type I membrane glycoprotein. This protein is about 35 kDa in mass and primarily expresses in epithelial tissues. It acts as a transmembrane receptor that plays a role in signal transduction due to its domain structure binding and activation processes on the cell surface. TROP2 exhibits high expression in several types of tumors making it a potential target for therapeutic antibodies like anti-TROP2 anti trop2 or anti-TROP-2.
Biological function summary

TROP2 mediates calcium signaling and cell proliferation. It functions by being a part of a signaling complex on the cell membrane which affects multiple cellular activities such as cell growth motility and survival. Its role in maintaining the integrity of the epithelial cell layer also shows TROP2's significance in normal tissue homeostasis. The interaction of TROP2 with the extracellular matrix and other cell surface proteins support the structural integrity and communication between cells.

Pathways

TROP2 plays a central role in the PI3K/AKT and MAPK signaling pathways that regulate cell growth and survival. Within these pathways TROP2 works in concert with proteins like ERK and AKT important for transmitting extracellular signals into appropriate cellular responses. These pathways are key regulators of many physiological processes including cell cycle progression and apoptosis prevention.

TROP2 is strongly associated with several types of cancers particularly epithelial cancers like breast and pancreatic cancer. Overexpression of TROP2 in tumors often correlates with poor prognosis and aggressive disease phenotypes. Interaction between TROP2 and proteins like EGFR in cancer cell signaling pathways amplifies tumor progression and metastasis. Understanding these associations makes TROP2 a significant target in developing therapeutic strategies for cancer treatment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May function as a growth factor receptor.
See full target information TACSTD2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com