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AB34606

HRP Anti-Trypsin Inhibitor antibody

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(1 Publication)

Rabbit Polyclonal Trypsin Inhibitor antibody - conjugated to HRP. Suitable for ELISA, Dot, WB and reacts with Soybean samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Soybean KTI3.

View Alternative Names

Trypsin inhibitor A, Kunitz-type trypsin inhibitor A, KTI3

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Soybean

Applications

Dot, WB, ELISA

applications

Immunogen

Full Length Protein corresponding to Soybean KTI3. The exact immunogen used to generate this antibody is proprietary information.

P01070

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Soybean": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/40000", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trypsin Inhibitor also known as Serine Protease Inhibitor Kazal-Type 1 (SPINK1) is a small protein with a mass of around 6 kilodaltons. It inhibits the activity of trypsin a serine protease by forming a stable complex which prevents the breakdown of protein substrates. This inhibitor is primarily expressed in pancreatic tissues as well as throughout the gastrointestinal tract. Its function is important in regulating the activity of digestive enzymes to prevent autodigestion of pancreatic cells.
Biological function summary

Trypsin Inhibitor plays a role in maintaining the balance of proteolytic activity in the pancreas. Inhibition of trypsin serves to protect tissue integrity by preventing premature activation of digestive enzymes. SPINK1 is not part of a larger protein complex but functions independently to bind with trypsin and other related proteases. This modulation of protease activity is essential for normal cellular homeostasis and digestive processes.

Pathways

Trypsin Inhibitor takes part in the proteolytic cascade that occurs in pancreatic cells. It acts within the balance-maintaining pathway between trypsinogen and trypsin where it inhibits premature conversion and activation of these enzymes. Related proteins include chymotrypsin and elastase which like trypsin are activated from their precursors in the digestive process. The Trypsin Inhibitor therefore ensures the safe regulation of these proteolytic enzymes.

Trypsin Inhibitor appears connected to pancreatitis and certain forms of pancreatic cancer. Mutations in the SPINK1 gene are associated with chronic pancreatitis where the regulated process of enzyme inhibition is disrupted leading to pancreatic tissue damage. Additionally overexpression of Trypsin Inhibitor has been noted in some pancreatic cancers potentially aiding in tumor progression by preventing enzyme-mediated destruction. The protein interacts with mutated forms of trypsin that may contribute to these disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Inhibition of trypsin.
See full target information KTI3

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 211:145-58 PubMed26438827

2015

Prion aggregate structure in yeast cells is determined by the Hsp104-Hsp110 disaggregase machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan O'Driscoll,Daniel Clare,Helen Saibil
View all publications

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