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AB306420

HRP Anti-UBA5 antibody [EPR11729]

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Rabbit Recombinant Monoclonal UBA5 antibody - conjugated to HRP.

View Alternative Names

UBE1DC1, UBA5, Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11729

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBA5 also known as Ubiquitin-like modifier-activating enzyme 5 is an E1 enzyme that plays a mechanical role in activating ubiquitin-fold modifier 1 (UFM1) for protein modification. It has a molecular mass of about 44 kDa and performs its function in the nucleus and cytoplasm within human cells. The expression of UBA5 is not limited to specific tissues; instead it shows widespread expression across many tissues supporting an important role in various cellular processes.
Biological function summary

UBA5 is implicated in the UFM1 conjugation system. This process modifies specific proteins and affects protein stability interaction and degradation. UBA5 operates in complex with UFC1 an E2 enzyme enabling the transfer of UFM1 to target proteins. Its activity is essential for maintaining cellular homeostasis particularly in the endoplasmic reticulum stress response through protein modification and regulation tasks.

Pathways

The UBA5-UFM1 axis integrates into the cellular stress response pathway and the endoplasmic reticulum-associated degradation (ERAD) pathway. Through these pathways UBA5 collaborates with proteins like UFL1 further connecting these pathways by tagging proteins with UFM1 to mark them for degradation or functional modification. The coordination between UBA5 and UFL1 supports efficient protein quality control and maintains cellular equilibrium under stress conditions.

The dysfunction or altered expression of UBA5 associates with neurodevelopmental disorders and some cancers. Changes in UBA5 activity can lead to imbalances in protein homeostasis contributing to pathogenic mechanisms in neurodevelopmental disorders where other proteins like EIF4A1 also play a role. In cancer alterations in UBA5 expression may influence tumor progression as seen in cases where UBA5 levels affect pathways that involve p53 an important tumor suppressor protein.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed : 15071506, PubMed : 18442052, PubMed : 20368332, PubMed : 25219498, PubMed : 26929408, PubMed : 27545674, PubMed : 27545681, PubMed : 27653677, PubMed : 30412706, PubMed : 30626644, PubMed : 34588452). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed : 20368332, PubMed : 26929408, PubMed : 27653677, PubMed : 30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed : 27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed : 29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed : 27653677, PubMed : 34588452). Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed : 30412706, PubMed : 32160526, PubMed : 35394863). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity).
See full target information UBA5
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