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Rabbit Recombinant Monoclonal UBC Linkage-specific K27 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.

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Images

Western blot - HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB237256), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

HRP

Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

UbiquitinExists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal UBC Linkage-specific K27 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Conjugation

HRP

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17034

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256), expandable thumbnail

    Western blot - HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)

    This blot was produced using a 4-12% Bis-Tris gel under the MES buffer system. The gel was run at 200V for 35 mins. before being transferred onto a nitrocellulose membrane at 30V for 70 mins. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab237256 (1/1000) overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    Samples:

    Lane 1. Di-Ubiquitin/Ub2 (Full Length) (20ng)

    Lane 2. Di-Ubiquitin/Ub2 (K11 Linked) (20ng)

    Lane 3. Di-Ubiquitin/Ub2 (K27 Linked) (20ng)

    Lane 4. Di-Ubiquitin/Ub2 (K29 Linked) (20ng)

    Lane 5. Di-Ubiquitin/Ub2 (K33 Linked ) (20ng)

    Lane 6. Di-Ubiquitin/Ub2 (K48 Linked) (20ng)

    Lane 7. Di-Ubiquitin/Ub2 (K6 Linked) (20ng)

    Lane 8. Di-Ubiquitin/Ub2 (K63 Linked) (20ng)

    All lanes: Western blot - HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)

    Predicted band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com