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Mouse Monoclonal UQCRC2 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - HRP Anti-UQCRC2 antibody [13G12AF12BB11] (AB197954), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-UQCRC2 antibody [13G12AF12BB11] (AB197954), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

2 products for Alternative Product

Target data

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).

Alternative names

Complex III subunit 2, Core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCRC2

Recommended products

Mouse Monoclonal UQCRC2 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG1
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Clone number
13G12AF12BB11
Purification technique
Affinity purification
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UQCRC2 also known as QCR2 is a subunit of the mitochondrial complex III also referred to as cytochrome bc1 complex. It has a molecular weight of approximately 48 kDa. This protein is located in the inner mitochondrial membrane across various tissue types playing an essential role in the mitochondrial electron transport chain. UQCRC2 facilitates the transfer of electrons from ubiquinol to cytochrome c which is an important step in cellular energy production.

Biological function summary

UQCRC2 participates in producing ATP which is the primary energy currency in cells. It forms part of the mitochondrial complex III a multi-subunit enzyme complex essential for efficient electron transfer and proton pumping. UQCRC2 contributes to establishing the proton gradient across the mitochondrial membrane which drives ATP synthesis through chemiosmotic processes. This function is critical for maintaining cellular energy homeostasis.

Pathways

The functionality of UQCRC2 links significantly to the oxidative phosphorylation and respiratory chain pathways. It ensures proper function in the oxidative phosphorylation pathway which generates the majority of ATP in aerobic organisms. UQCRC2 is associated with proteins such as cytochrome b and cytochrome c1 which collaborate in the electron transport chain ensuring the efficiency of the process and integrity of energy conversion.

Associated diseases and disorders

The aberrant function of UQCRC2 associates with mitochondrial disorders and certain cancers. Mutations affecting UQCRC2 function can lead to mitochondrial diseases characterized by energy production deficits resulting in neuromuscular and metabolic complications. Additionally studies have linked altered UQCRC2 expression to cancer where it may interact with proteins like p53 playing roles in metabolic reprogramming of cells.

Product promise

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2 product images

  • Western blot - HRP Anti-UQCRC2 antibody [13G12AF12BB11] (ab197954), expandable thumbnail

    Western blot - HRP Anti-UQCRC2 antibody [13G12AF12BB11] (ab197954)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197954 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406

    All lanes: Western blot - HRP Anti-UQCRC2 antibody [13G12AF12BB11] (ab197954) at 1/5000 dilution

    Lane 1: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Lane 2: Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

    Exposure time: 20min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-UQCRC2 antibody [13G12AF12BB11] (ab197954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-UQCRC2 antibody [13G12AF12BB11] (ab197954)

    IHC image of UQCRC2 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197954, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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