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AB198948

HRP Anti-VAMP2 antibody [EPR12790]

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(1 Publication)

Rabbit Recombinant Monoclonal VAMP2 antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

SYB2, VAMP2, Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2

1 Images
Western blot - HRP Anti-VAMP2 antibody [EPR12790] (AB198948)
  • WB

Lab

Western blot - HRP Anti-VAMP2 antibody [EPR12790] (AB198948)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198948 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-VAMP2 antibody [EPR12790] (ab198948) at 1/5000 dilution

All lanes:

Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg

Predicted band size: 13 kDa

Observed band size: 18 kDa

true

Exposure time: 10s

  • Carrier free

    Anti-VAMP2 antibody [EPR12790] - BSA and Azide free

  • Unconjugated

    Anti-VAMP2 antibody [EPR12790]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-VAMP2 antibody [EPR12790]

  • 578 PE

    PE Anti-VAMP2 antibody [EPR12790]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12790

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein 2 (VAMP2) also known as synaptobrevin 2 is a member of the SNARE protein family with a molecular mass of approximately 13 kDa. VAMP2 plays an important role in vesicle trafficking and membrane fusion particularly in the nervous system. It is prominently expressed in the brain where it facilitates neurotransmitter release by forming SNARE complexes essential for synaptic vesicle fusion with the presynaptic membrane.
Biological function summary

VAMP2 interacts with other SNARE proteins such as synaptosomal-associated protein 25 (SNAP-25) and syntaxin-1 to form stable complexes necessary for synaptic vesicle docking and exocytosis. As part of the SNARE complex VAMP2 contributes to the precise regulation of neurotransmitter release in neurons which is critical for synaptic transmission and plasticity. Its function is vital for the communication between neurons and proper brain function.

Pathways

VAMP2 plays a significant role in the synaptic vesicle cycle and neurotransmitter release pathways. It is central to the exocytosis pathway where it interacts with Rab3a a small GTPase that regulates vesicle docking. By participating in these pathways VAMP2 ensures efficient neurotransmission and contributes to neuronal communication processes. VAMP2's interaction with related proteins like SNAP-25 and syntaxin-1 highlights its importance in these synaptic processes.

VAMP2 has associations with conditions such as epilepsy and neurodegenerative diseases. Alterations in VAMP2 expression or function can disrupt normal neurotransmitter release impacting synaptic function and leading to neurological symptoms. VAMP2's relationship with SNAP-25 and syntaxin-1 further implicates it in these disorders as these proteins collectively regulate neural communication. Understanding VAMP2's role in these illnesses aids in the development of potential therapeutic strategies targeting SNARE complex dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (By similarity) (PubMed : 30929742). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
See full target information VAMP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Eye and vision (London, England) 9:4 PubMed35101125

2022

SUV39H1 regulates corneal epithelial wound healing via H3K9me3-mediated repression of p27.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Weiwei Chen,Shanshan Jin,Guangying Luo,Xia Jing,Qi Liu,Peter S Reinach,Jia Qu,Dongsheng Yan
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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