AB185063

HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal VDAC1/Porin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

VDAC, VDAC1, Non-selective voltage-gated ion channel VDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1

1 Images

Lab

Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB185063)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185063 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063) at 1/5000 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 5:

Kidney (Rat) Tissue Lysate at 10 µg

Lane 6:

Kidney (Human) Tissue Lysate - adult normal tissue at 10 µg

Observed band size: 35 kDa

true

Exposure time: 4min

578 PE

PE Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
660 APC

APC Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control
Unconjugated

Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control
Carrier free

Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10852(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The VDAC1/Porin and VDAC2 proteins also known as voltage-dependent anion-selective channel 1 and 2 serve as important pore-forming proteins within the mitochondrial outer membrane. Each VDAC protein has a molecular mass of about 30-35 kDa. Commonly referred to as the 'porin hub' due to their central role in metabolite exchange VDAC1 and VDAC2 facilitate the transport of ions and small molecules between the mitochondria and cytoplasm regulating energy production and cellular metabolism. They express widely across various tissues highlighting their essential role in cellular functions.

Biological function summary

Voltage-dependent anion channels like VDAC1/Porin and VDAC2 modulate the exchange of ions and metabolic substrates playing a significant role in apoptosis and the maintenance of mitochondrial functions. As integral parts of mitochondrial complexes they interact with proteins such as hexokinase which anchors to VDAC1 and influences glycolytic flux linking energy production with apoptotic signaling pathways. This positions VDAC proteins as key regulators of cellular energy homeostasis.

Pathways

VDAC1/Porin and VDAC2 engage prominently in the apoptotic and mitochondrial permeability transition pathways. In the apoptosis pathway they interact with anti-apoptotic proteins like Bcl-xl and pro-apoptotic members like Bax highlighting their dual role in cell survival and death. These interactions highlight the proteins’ contribution to mitochondrial outer membrane permeabilization (MOMP) a critical event in the release of cytochrome c and subsequent apoptosome formation further integrating them into broader signaling networks.

Dysfunctions in VDAC1/Porin and VDAC2 link to neurodegenerative diseases and cancer. Aberrant VDAC1 activity connects with Alzheimer's disease due to its role in mitochondrial dysfunction affecting amyloid-beta peptide accumulation and neuronal cell death. In cancer VDAC overexpression associates with altered cellular metabolism and resistance to apoptosis positioning these proteins as potential therapeutic targets. Interactions with proteins such as hexokinase and Bcl-2 family members further emphasize their role in these pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 30061676, PubMed : 8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed : 10661876, PubMed : 18755977, PubMed : 8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed : 18755977, PubMed : 8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed : 18755977, PubMed : 31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed : 32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed : 15033708, PubMed : 25296756). May mediate ATP export from cells (PubMed : 30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed : 20230784).. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

See full target information VDAC1

Additional targets

Voltage-dependent anion-selective channel protein 2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

ACS infectious diseases 10:3853-3867 PubMed39475219

2024

Modulators of the Hop-HSP90 Protein-Protein Interaction Disrupt KSHV Lytic Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Michael O Okpara,Michaelone C Vaaltyn,Jessica L Watson,Mahama Alhassan,Fernando Albericio,Beatriz G de la Torre,David J Clarke,Clinton G L Veale,Adrienne L Edkins

Mitochondrion 63:43-50 PubMed35032706

2022

Dysregulation of mitochondrial complexes and dynamics by chronic cigarette smoke exposure Utilizing MitoQC reporter mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qixin Wang,Hoshang Unwalla,Irfan Rahman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB185063)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

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