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Rabbit Recombinant Monoclonal Vimentin antibody - conjugated to HRP. Cytoskeleton marker. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB194718), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB194718), expandable thumbnail
  • Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB194718), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/500
Notes

ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Version

1 product for Alternative Product

Target data

Function

Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Vimentin, VIM

Recommended products

Rabbit Recombinant Monoclonal Vimentin antibody - conjugated to HRP. Cytoskeleton marker. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3776
Purification technique
Affinity purification Protein A
Dissociation constant
1.1 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718), expandable thumbnail

    Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718)

    ab194718 was shown to specifically react with VIM in wild-type HAP1 cells as signal was lost in VIM knockout cells. Wild-type and VIM knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab194718 and Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    All lanes: Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: VIM knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 53 kDa

    Exposure time: 150s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718)

    IHC image of Vimentin staining in a section of formalin-fixed paraffin-embedded human breast (Fibroadenoma)*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194718, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718), expandable thumbnail

    Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194718 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718) at 1/1000 dilution

    Lane 1: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035) at 10 µg

    Lane 2: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Lane 3: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa

    Observed band size: 54 kDa

    Exposure time: 2s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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