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Rabbit Recombinant Monoclonal Vinculin antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - HRP Anti-Vinculin antibody [EPR8185] - Loading Control (AB323345), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Alternative names

Vinculin, Metavinculin, MV, VCL

Rabbit Recombinant Monoclonal Vinculin antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8185
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - HRP Anti-Vinculin antibody [EPR8185] - Loading Control (ab323345), expandable thumbnail

    Western blot - HRP Anti-Vinculin antibody [EPR8185] - Loading Control (ab323345)

    Vinculin Western blot staining using rabbit Anti-Vinculin antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Performed under reducing conditions.

    In Western blot, ab323345 was shown to bind specifically to Vinculin. Target of interest was observed at 123 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in Vinculin knockout cell line (lane 2) (lane 2, knockout cell line Human VCL (Vinculin) knockout HeLa cell line ab265580.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - HRP Anti-Vinculin antibody [EPR8185] - Loading Control (ab323345) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Vincin knockout HeLa whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Observed band size: 123 kDa, 36 kDa

    Exposure time: 3.25s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com