AB195030

HRP Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb
Recombinant
What is this?

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Rabbit Recombinant Monoclonal Von Willebrand Factor antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

1 Images

Lab

Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (AB195030)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195030 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (ab195030) at 1/5000 dilution

All lanes:

Human Plasma Total Protein Lysate at 10 µg

Predicted band size: 309 kDa

Observed band size: 230 kDa

true

Exposure time: 20min

Unconjugated

Anti-Von Willebrand Factor antibody [EPSISR15]
Carrier free

Anti-Von Willebrand Factor antibody [EPSISR15] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Von Willebrand Factor antibody [EPSISR15]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Von Willebrand Factor antibody [EPSISR15]
660 APC

APC Anti-Von Willebrand Factor antibody [EPSISR15]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Von Willebrand Factor antibody [EPSISR15]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR15

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/hrp-rabbit-igg-monoclonal-epr25a-isotype-control-ab199507'>ab199507</a> - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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You may be interested in:

AB108918

Human Von Willebrand Factor ELISA Kit (VWF)

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View product

Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene VWF is important for maintaining hemostasis. It promotes platelet adhesion at sites of vascular injury by forming a molecular bridge between the sub-endothelial collagen matrix and the platelet-surface receptor complex GPIb-IX-V. Additionally, it acts as a chaperone for coagulation factor VIII, delivering it to the injury site, stabilizing its heterodimeric structure, and protecting it from premature clearance from plasma. This supplementary information is collated from multiple sources and compiled automatically.

See full target information VWF

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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primary-antibodies

alexa-fluor-647-von-willebrand-factor-antibody-epsisr15-ab195029

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Von Willebrand Factor antibody [EPSISR15]

Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (AB195030)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

You may be interested in:

Human Von Willebrand Factor ELISA Kit (VWF)

Why is this recommended?

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Product promise

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