Rabbit Recombinant Monoclonal Von Willebrand Factor antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.
Images
![Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (AB195030), expandable thumbnail](https://content.abcam.com/products/images/hrp-von-willebrand-factor-antibody-epsisr15-ab195030--western-blot-img66627.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (AB195030)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Not recommended | Tested |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
3 products for Alternative Version
Target data
Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Alternative names
F8VWF, VWF, von Willebrand factor, vWF
Recommended products
Rabbit Recombinant Monoclonal Von Willebrand Factor antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPSISR15
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (ab195030), expandable thumbnail](https://content.abcam.com/products/images/hrp-von-willebrand-factor-antibody-epsisr15-ab195030--western-blot-img66627.jpg "Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (ab195030)")
Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (ab195030)
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195030 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Von Willebrand Factor antibody [EPSISR15] (ab195030) at 1/5000 dilution
All lanes: Human Plasma Total Protein Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 309 kDa
Observed band size: 230 kDa
Exposure time: 20min
Downloads
Product protocols
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