Rabbit Monoclonal HSC70 Interacting Protein HIP antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Images
![Western blot - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005), expandable thumbnail](https://content.abcam.com/products/images/hsc70-interacting-protein-hip-antibody-ep3264-ab76005--western-blot-img59292.gif "Western blot - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (AB76005)")
Publications
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- Frontiers in oncology 11:6726842021Exosomal miR-183-5p Shuttled by M2 Polarized Tumor-Associated Macrophage Promotes the Development of Colon Cancer Targeting THEM4 Mediated PI3K/AKT and NF-κB Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShangxin Zhang,Deguan Li,Min Zhao,Fei Yang,Changye Sang,Changhong Yan,Zhenjun Wang,Yongxiang LiPubMed 34249713
- Biomaterials science 7:4310-43242019Use of l-pNIPAM hydrogel as a 3D-scaffold for intestinal crypts and stem cell tissue engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRasha H Dosh,Nicola Jordan-Mahy,Christopher Sammon,Christine L Le MaitrePubMed 31410428
- Biomedicine & pharmacotherapy = Biomedecine & phar 107:1210-12172018Toll-like receptor 4 shRNA attenuates lipopolysaccharide-induced epithelial-mesenchymal transition of intrahepatic biliary epithelial cells in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShifang Tang et. al.PubMed 30257335
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/250.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity).
Alternative names
AAG2, FAM10A1, HIP, SNC6, ST13, Hsc70-interacting protein, Hip, Aging-associated protein 2, Progesterone receptor-associated p48 protein, Protein FAM10A1, Putative tumor suppressor ST13, Renal carcinoma antigen NY-REN-33, Suppression of tumorigenicity 13 protein
Recommended products
Rabbit Monoclonal HSC70 Interacting Protein HIP antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP3264
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)
All lanes: Western blot - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005) at 1/100000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: SW480 cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005), expandable thumbnail](https://content.abcam.com/products/images/hsc70-interacting-protein-hip-antibody-ep3264-ab76005--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117685.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)
ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005), expandable thumbnail](https://content.abcam.com/products/images/hsc70-interacting-protein-hip-antibody-ep3264-ab76005--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134480.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)
ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human normal colon by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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