Rabbit Polyclonal HSD11B1 antibody. Suitable for WB and reacts with Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human HSD11B1 aa 50-100.
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- Genes and immunity 24:52-562023Human lung carcinomas synthesize immunoregulatory glucocorticoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVerena M Merk,Leonie Grob,Achim Fleischmann,Thomas BrunnerPubMed 36653475
- Cell reports 39:1107852022Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbigail P Leinroth,Anthony J Mirando,Douglas Rouse,Yoshihiko Kobayahsi,Purushothama Rao Tata,Helen E Rueckert,Yihan Liao,Jason T Long,Joe V Chakkalakal,Matthew J HiltonPubMed 35545045
- The Journal of physiology 599:4705-47242021Impact of maternal late gestation undernutrition on surfactant maturation, pulmonary blood flow and oxygen delivery measured by magnetic resonance imaging in the sheep fetus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaqi Ren,Jack R T Darby,Mitchell C Lock,Stacey L Holman,Brahmdeep S Saini,Emma L Bradshaw,Sandra Orgeig,Sunthara R Perumal,Michael D Wiese,Christopher K Macgowan,Mike Seed,Janna L MorrisonPubMed 34487347
- Neurobiology of disease 157:1054232021Proteomic signature of the Dravet syndrome in the genetic Scn1a-A1783V mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNina Miljanovic,Stefanie M Hauck,R Maarten van Dijk,Valentina Di Liberto,Ali Rezaei,Heidrun PotschkaPubMed 34144125
- Frontiers in pharmacology 12:59443720215-Bis-(2,6-difluoro-benzylidene) Cyclopentanone Acts as a Selective 11β-Hydroxysteroid Dehydrogenase one Inhibitor to Treat Diet-Induced Nonalcoholic Fatty Liver Disease in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongguo Guan,Yiyan Wang,Huitao Li,Qiqi Zhu,Xiaoheng Li,Guang Liang,Ren-Shan GePubMed 33912032
- Nutrients 12:2020Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvana Elaković et. al.PubMed 33198224
- Cell death discovery 6:242020HSD11B1 is upregulated synergistically by IFNγ and TNFα and mediates TSG-6 expression in human UC-MSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeiqing Huang et. al.PubMed 32328292
- Journal of medicinal chemistry 62:6925-69402019Mechanistic Insight on the Mode of Action of Colletoic Acid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaotao Ling,Darcie J Miller,Walter H Lang,Elizabeth Griffith,Adaris Rodriguez-Cortes,Ikbale El Ayachi,Gustavo Palacios,Jaeki Min,Gustavo Miranda-Carboni,Richard E Lee,Fatima RivasPubMed 31294974
- Frontiers in pharmacology 10:7372019Analyze and Identify Peiminine Target EGFR Improve Lung Function and Alleviate Pulmonary Fibrosis to Prevent Exacerbation of Chronic Obstructive Pulmonary Disease by Phosphoproteomics Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyao Ma et. al.PubMed 31333459
- Nutrients 11:2019Glycyrrhetinic Acid Improves Insulin-Response Pathway by Regulating the Balance between the Ras/MAPK and PI3K/Akt Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Zhang et. al.PubMed 30871060
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HSD11B1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P28845
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Cat | Predicted |
| Cow | Predicted |
| Cynomolgus monkey | Predicted |
| Dog | Predicted |
| Hamster | Predicted |
| Macaque monkey | Predicted |
| Monkey | Predicted |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Hamster, Cow, Cat, Dog, Monkey, Cynomolgus monkey, Macaque monkey | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Controls the reversible conversion of biologically active glucocorticoids such as cortisone to cortisol, and 11-dehydrocorticosterone to corticosterone in the presence of NADP(H) (PubMed:10497248, PubMed:12460758, PubMed:14973125, PubMed:15152005, PubMed:15280030, PubMed:17593962, PubMed:21453287, PubMed:27927697, PubMed:30902677). Participates in the corticosteroid receptor-mediated anti-inflammatory response, as well as metabolic and homeostatic processes (PubMed:10497248, PubMed:12414862, PubMed:15152005, PubMed:21453287). Plays a role in the secretion of aqueous humor in the eye, maintaining a normotensive, intraocular environment (PubMed:11481269). Bidirectional in vitro, predominantly functions as a reductase in vivo, thereby increasing the concentration of active glucocorticoids (PubMed:10497248, PubMed:11481269, PubMed:12414862, PubMed:12460758). It has broad substrate specificity, besides glucocorticoids, it accepts other steroid and sterol substrates (PubMed:15095019, PubMed:15152005, PubMed:17593962, PubMed:21453287). Interconverts 7-oxo- and 7-hydroxy-neurosteroids such as 7-oxopregnenolone and 7beta-hydroxypregnenolone, 7-oxodehydroepiandrosterone (3beta-hydroxy-5-androstene-7,17-dione) and 7beta-hydroxydehydroepiandrosterone (3beta,7beta-dihydroxyandrost-5-en-17-one), among others (PubMed:17593962). Catalyzes the stereo-specific conversion of the major dietary oxysterol, 7-ketocholesterol (7-oxocholesterol), into the more polar 7-beta-hydroxycholesterol metabolite (PubMed:15095019, PubMed:15152005). 7-oxocholesterol is one of the most important oxysterols, it participates in several events such as induction of apoptosis, accumulation in atherosclerotic lesions, lipid peroxidation, and induction of foam cell formation (PubMed:15095019). Mediates the 7-oxo reduction of 7-oxolithocholate mainly to chenodeoxycholate, and to a lesser extent to ursodeoxycholate, both in its free form and when conjugated to glycine or taurine, providing a link between glucocorticoid activation and bile acid metabolism (PubMed:21453287). Catalyzes the synthesis of 7-beta-25-dihydroxycholesterol from 7-oxo-25-hydroxycholesterol in vitro, which acts as a ligand for the G-protein-coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2) and may thereby regulate immune cell migration (PubMed:30902677).
Alternative names
HSD11, HSD11L, SDR26C1, HSD11B1, 11-beta-hydroxysteroid dehydrogenase 1, 11-DH, 11-beta-HSD1, 7-oxosteroid reductase, Corticosteroid 11-beta-dehydrogenase isozyme 1, Short chain dehydrogenase/reductase family 26C member 1
Recommended products
Rabbit Polyclonal HSD11B1 antibody. Suitable for WB and reacts with Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human HSD11B1 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HSD11B1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P28845
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab39364 is an affinity purified IgG
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
This image is courtesy of a customer review submitted by Rob Cordery-Cotter. Experimental work performed by Megan A. Walton.Western blot - Anti-HSD11B1 antibody (ab39364)
Western blot analysis of Sheep liver tissue lysate (5μg/lane) labeling HSD11B1 with ab39364 at 1/500 (16 hours at 4°C). An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
All lanes: Western blot - Anti-HSD11B1 antibody (ab39364)
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 37 kDa
Exposure time: 1min
Western blot - Anti-HSD11B1 antibody (ab39364)
HSD11B1 contains a number of potential glycosylation sites (SwissProt) which may explain the higher migration pattern observed.
All lanes: Western blot - Anti-HSD11B1 antibody (ab39364) at 1 µg/mL
All lanes: Human liver tissue lysate - total protein (ab29889) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Exposure time: 16min
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