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AB157223

Anti-HSD11B1 antibody [EPR9407(2)]

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(5 Publications)

Rabbit Recombinant Monoclonal HSD11B1 antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

HSD11, HSD11L, SDR26C1, HSD11B1, 11-beta-hydroxysteroid dehydrogenase 1, 11-DH, 11-beta-HSD1, 7-oxosteroid reductase, Corticosteroid 11-beta-dehydrogenase isozyme 1, Short chain dehydrogenase/reductase family 26C member 1

4 Images
Flow Cytometry (Intracellular) - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HSD11B1 with Purified ab157223 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HSD11B1 with purified ab157223 at 1/1500 dilution (0.172 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)

Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HSD11B1 with Purified ab157223 at 1 : 50 dilution (5.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)
  • WB

Unknown

Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (AB157223)

All lanes:

Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223) at 1/10000 dilution

All lanes:

Human liver lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 32 kDa

false

  • Carrier free

    Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9407(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Controls the reversible conversion of biologically active glucocorticoids such as cortisone to cortisol, and 11-dehydrocorticosterone to corticosterone in the presence of NADP(H) (PubMed : 10497248, PubMed : 12460758, PubMed : 14973125, PubMed : 15152005, PubMed : 15280030, PubMed : 17593962, PubMed : 21453287, PubMed : 27927697, PubMed : 30902677). Participates in the corticosteroid receptor-mediated anti-inflammatory response, as well as metabolic and homeostatic processes (PubMed : 10497248, PubMed : 12414862, PubMed : 15152005, PubMed : 21453287). Plays a role in the secretion of aqueous humor in the eye, maintaining a normotensive, intraocular environment (PubMed : 11481269). Bidirectional in vitro, predominantly functions as a reductase in vivo, thereby increasing the concentration of active glucocorticoids (PubMed : 10497248, PubMed : 11481269, PubMed : 12414862, PubMed : 12460758). It has broad substrate specificity, besides glucocorticoids, it accepts other steroid and sterol substrates (PubMed : 15095019, PubMed : 15152005, PubMed : 17593962, PubMed : 21453287). Interconverts 7-oxo- and 7-hydroxy-neurosteroids such as 7-oxopregnenolone and 7beta-hydroxypregnenolone, 7-oxodehydroepiandrosterone (3beta-hydroxy-5-androstene-7,17-dione) and 7beta-hydroxydehydroepiandrosterone (3beta,7beta-dihydroxyandrost-5-en-17-one), among others (PubMed : 17593962). Catalyzes the stereo-specific conversion of the major dietary oxysterol, 7-ketocholesterol (7-oxocholesterol), into the more polar 7-beta-hydroxycholesterol metabolite (PubMed : 15095019, PubMed : 15152005). 7-oxocholesterol is one of the most important oxysterols, it participates in several events such as induction of apoptosis, accumulation in atherosclerotic lesions, lipid peroxidation, and induction of foam cell formation (PubMed : 15095019). Mediates the 7-oxo reduction of 7-oxolithocholate mainly to chenodeoxycholate, and to a lesser extent to ursodeoxycholate, both in its free form and when conjugated to glycine or taurine, providing a link between glucocorticoid activation and bile acid metabolism (PubMed : 21453287). Catalyzes the synthesis of 7-beta-25-dihydroxycholesterol from 7-oxo-25-hydroxycholesterol in vitro, which acts as a ligand for the G-protein-coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2) and may thereby regulate immune cell migration (PubMed : 30902677).
See full target information HSD11B1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 11:103 PubMed40307230

2025

The role of alpha-synuclein in synucleinopathy: Impact on lipid regulation at mitochondria-ER membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Peter A Barbuti,Cristina Guardia-Laguarta,Taekyung Yun,Zena K Chatila,Xena Flowers,Chantel Wong,Bruno F R Santos,Simone B Larsen,James S Lotti,Nobutaka Hattori,Elizabeth Bradshaw,Ulf Dettmer,Saranna Fanning,Vilas Menon,Hasini Reddy,Andrew F Teich,Rejko Krüger,Estela Area-Gomez,Serge Przedborski

Frontiers in bioengineering and biotechnology 12:1422761 PubMed39036559

2024

11β hydroxysteroid dehydrogenase type 1 transgenic mesenchymal stem cells attenuate inflammation in models of sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul Y Mahida,Zhengqiang Yuan,Krishna K Kolluri,Aaron Scott,Dhruv Parekh,Rowan S Hardy,Michael A Matthay,Gavin D Perkins,Sam M Janes,David R Thickett

BioTechniques 73:113-122 PubMed36066013

2022

Ancient DNA analysis from epoxy resin Biodur-embedded bones.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Lena Flux,Michael Schultz,Susanne Hummel

Frontiers in bioengineering and biotechnology 10:953034 PubMed36091434

2022

Effects of knockout and overexpression on local cortisol production and differentiation of mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Angelique Kragl,Janosch Schoon,Ana Tzvetkova,Christoph Wenzel,Martina Blaschke,Wolfgang Böcker,Heide Siggelkow,Mladen V Tzvetkov

International journal of molecular sciences 23: PubMed35682649

2022

RNA-Seq Transcriptome Analysis of Differentiated Human Oligodendrocytic MO3.13 Cells Shows Upregulation of Genes Involved in Myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Głowacka,Ewa Kilańczyk,Małgorzata Maksymowicz,Małgorzata Zawadzka,Wiesława Leśniak,Anna Filipek
View all publications
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Product promise

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