Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (AB175049)

This data was developed using ab157223, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HSD11B1 with Purified ab157223 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (AB175049)

This data was developed using ab157223, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HSD11B1 with purified ab157223 at 1/1500 dilution (0.172 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (AB175049)

This data was developed using ab157223, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HSD11B1 with Purified ab157223 at 1 : 50 dilution (5.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Unknown

Western blot - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (AB175049)

All lanes:

Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (<a href='/en-us/products/primary-antibodies/hsd11b1-antibody-epr94072-ab157223'>ab157223</a>) at 1/10000 dilution

All lanes:

Human liver lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 32 kDa

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