Rabbit Polyclonal HSD11B2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Horse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HSD11B2 aa 150-250 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Cells 11:2022Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiselott Källsten,Paula Pierozan,Jonathan W Martin,Oskar KarlssonPubMed 36230992
- Cells 11:2022Aldosterone Increases Vascular Permeability in Rat Skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichal Aleksiejczuk,Anna Gromotowicz-Poplawska,Natalia Marcinczyk,Joanna Stelmaszewska,Janusz Dzieciol,Ewa ChabielskaPubMed 36078114
- Clinical science (London, England : 1979) 133:2449-24612019Relationship between the renin-angiotensin-aldosterone system and renal Kir5.1 channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna D Manis,Oleg Palygin,Sherif Khedr,Vladislav Levchenko,Matthew R Hodges,Alexander StaruschenkoPubMed 31799617
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HSD11B2 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P80365
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Rat | Tested | Expected |
| Horse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Horse | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Horse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Catalyzes the conversion of biologically active 11beta-hydroxyglucocorticoids (11beta-hydroxysteroid) such as cortisol, to inactive 11-ketoglucocorticoids (11-oxosteroid) such as cortisone, in the presence of NAD(+) (PubMed:10497248, PubMed:12788846, PubMed:17314322, PubMed:22796344, PubMed:27927697, PubMed:30902677, PubMed:33387577, PubMed:7859916, PubMed:8538347). Functions as a dehydrogenase (oxidase), thereby decreasing the concentration of active glucocorticoids, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids (PubMed:10497248, PubMed:12788846, PubMed:17314322, PubMed:33387577, PubMed:7859916). Plays an important role in maintaining glucocorticoids balance during preimplantation and protects the fetus from excessive maternal corticosterone exposure (By similarity). Catalyzes the oxidation of 11beta-hydroxytestosterone (11beta,17beta-dihydroxyandrost-4-ene-3-one) to 11-ketotestosterone (17beta-hydroxyandrost-4-ene-3,11-dione), a major bioactive androgen (PubMed:22796344, PubMed:27927697). Catalyzes the conversion of 11beta-hydroxyandrostenedione (11beta-hydroxyandrost-4-ene-3,17-dione) to 11-ketoandrostenedione (androst-4-ene-3,11,17-trione), which can be further metabolized to 11-ketotestosterone (PubMed:27927697). Converts 7-beta-25-dihydroxycholesterol to 7-oxo-25-hydroxycholesterol in vitro (PubMed:30902677). 7-beta-25-dihydroxycholesterol (not 7-oxo-25-hydroxycholesterol) acts as a ligand for the G-protein-coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2) and may thereby regulate immune cell migration (PubMed:30902677). May protect ovulating oocytes and fertilizing spermatozoa from the adverse effects of cortisol (By similarity).
Alternative names
HSD11K, SDR9C3, HSD11B2, 11-beta-hydroxysteroid dehydrogenase type 2, 11-DH2, 11-beta-HSD2, 11-beta-hydroxysteroid dehydrogenase type II, Corticosteroid 11-beta-dehydrogenase isozyme 2, NAD-dependent 11-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 9C member 3, 11-HSD type II, 11-beta-HSD type II
Recommended products
Rabbit Polyclonal HSD11B2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Horse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HSD11B2 aa 150-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HSD11B2 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P80365
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
Western blot - Anti-HSD11B2 antibody (ab203132)
All lanes: Western blot - Anti-HSD11B2 antibody (ab203132) at 1/1000 dilution
All lanes: Mouse kidney lysates
SecondaryAll lanes: Conjugated secondary antibody at 1/20000 dilution
Predicted band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B2 antibody (ab203132)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling HSD11B2 with ab203132 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B2 antibody (ab203132)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling HSD11B2 with ab203132 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B2 antibody (ab203132)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling HSD11B2 with ab203132 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B2 antibody (ab203132)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded horse kidney tissue labeling HSD11B2 with ab203132 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B2 antibody (ab203132)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded horse kidney tissue labeling HSD11B2 with ab203132 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B2 antibody (ab203132)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling HSD11B2 with ab203132 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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