Rabbit Recombinant Monoclonal HSD17B1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (AB248715), expandable thumbnail](https://content.abcam.com/products/images/hsd17b1-antibody-ep1681y-bsa-and-azide-free-ab248715--western-blot-img57468.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (AB248715)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (AB248715), expandable thumbnail](https://content.abcam.com/products/images/hsd17b1-antibody-ep1681y-bsa-and-azide-free-ab248715--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102578.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (AB248715)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2) (PubMed:8994190). Also has 20-alpha-HSD activity. Uses preferentially NADH.
Alternative names
E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1, HSD17B1, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Estradiol 17-beta-dehydrogenase 1, Placental 17-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 28C member 1, 20-alpha-HSD
Recommended products
Rabbit Recombinant Monoclonal HSD17B1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1681Y
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248715 is the carrier-free version of Anti-HSD17B1 antibody [EP1681Y] ab134193.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (ab248715), expandable thumbnail](https://content.abcam.com/products/images/hsd17b1-antibody-ep1681y-bsa-and-azide-free-ab248715--western-blot-img57468.jpg "Western blot - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (ab248715)")
Western blot - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (ab248715)
This data was developed using Anti-HSD17B1 antibody [EP1681Y] ab134193, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HSD17B1 antibody [EP1681Y] (Anti-HSD17B1 antibody [EP1681Y] ab134193) at 1/500 dilution
All lanes: Human placenta lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (ab248715), expandable thumbnail](https://content.abcam.com/products/images/hsd17b1-antibody-ep1681y-bsa-and-azide-free-ab248715--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102578.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (ab248715)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1681Y] - BSA and Azide free (ab248715)
This data was developed using Anti-HSD17B1 antibody [EP1681Y] ab134193, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling HSD17B1 with Anti-HSD17B1 antibody [EP1681Y] ab134193 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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