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Rabbit Recombinant Monoclonal HSD17B1 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 17 publications.

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Images

Western blot - Anti-HSD17B1 antibody [EP1682Y] (AB51045), expandable thumbnail
  • Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (AB51045), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (AB51045), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HSD17B1 antibody [EP1682Y] (AB51045), expandable thumbnail

Publications

  • Frontiers in endocrinology 14:12682482023
    Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Mengxue Zheng,Claus Yding Andersen,Frida Roikjer Rasmussen,Jesús Cadenas,Søren Tvorup Christensen,Linn Salto Mamsen
    PubMed 37964966
  • The Journal of clinical endocrinology and metabolism 107:e3374-e33832022
    Intrafollicular Concentrations of the Oocyte-secreted Factors GDF9 and BMP15 Vary Inversely in Polycystic Ovaries.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Stine Gry Kristensen,Ajay Kumar,Linn Salto Mamsen,Bhanu Kalra,Susanne Elisabeth Pors,Jane Alrø Bøtkjær,Kirsten Tryde Macklon,Jens Fedder,Erik Ernst,Kate Hardy,Stephen Franks,Claus Yding Andersen
    PubMed 35511085

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

2 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2) (PubMed:8994190). Also has 20-alpha-HSD activity. Uses preferentially NADH.

      Alternative names

      17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Estradiol 17-beta-dehydrogenase 1, Placental 17-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 28C member 1, 20-alpha-HSD, SDR28C1, EDHB17, EDH17B1, E17KSR, EDH17B2, HSD17B1

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      Rabbit Recombinant Monoclonal HSD17B1 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 17 publications.

      Alternative names

      17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Estradiol 17-beta-dehydrogenase 1, Placental 17-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 28C member 1, 20-alpha-HSD, SDR28C1, EDHB17, EDH17B1, E17KSR, EDH17B2, HSD17B1

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EP1682Y

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      4 product images

      • Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045), expandable thumbnail

        Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

        All lanes: Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution

        All lanes: Human placenta tissue lysate at 10 µg

        Secondary

        All lanes: Goat anti-Rabbit HRP labeled at 1/2000 dilution

        Predicted band size: 35 kDa

        Observed band size: 35 kDa

      • Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (ab51045), expandable thumbnail

        Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

        Purified ab51045 at 1/50 dilution (2μg) immunoprecipitating HSD17B1 in Human placenta lysate.
        Lane 1 (input): Human placenta lysate 10μg
        Lane 2 (+): ab51045 + Human placenta lysate.
        Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab51045 in Human placenta lysate.
        VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
        Blocking Buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM/TBST.
        Observed band size: 34 kDa

        All lanes: Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

        Predicted band size: 35 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (ab51045), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

        ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Flow Cytometry (Intracellular) - Anti-HSD17B1 antibody [EP1682Y] (ab51045), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

        Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com