Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (AB247308)

This data was developed using ab51045, the same antibody clone in a different buffer formulation.

Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (AB247308)

This data was developed using ab51045, the same antibody clone in a different buffer formulation.

ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (AB247308)

This data was developed using ab51045, the same antibody clone in a different buffer formulation.
Purified ab51045 at 1/50 dilution (2μg) immunoprecipitating HSD17B1 in Human placenta lysate.
Lane 1 (input) : Human placenta lysate 10μg
Lane 2 (+) : ab51045 + Human placenta lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab51045 in Human placenta lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 34 kDa

All lanes:

Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (<a href='/en-us/products/primary-antibodies/hsd17b1-antibody-ep1682y-ab51045'>ab51045</a>)

Predicted band size: 35 kDa

false

• WB

Unknown

Western blot - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (AB247308)

This data was developed using ab51045, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HSD17B1 antibody [EP1682Y] (<a href='/en-us/products/primary-antibodies/hsd17b1-antibody-ep1682y-ab51045'>ab51045</a>) at 1/100000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false