Rabbit Polyclonal Hsd17b11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B11 aa 200 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HSD17B11 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NBQ5
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Can convert androstan-3-alpha,17-beta-diol (3-alpha-diol) to androsterone in vitro, suggesting that it may participate in androgen metabolism during steroidogenesis. May act by metabolizing compounds that stimulate steroid synthesis and/or by generating metabolites that inhibit it. Has no activity toward DHEA (dehydroepiandrosterone), or A-dione (4-androste-3,17-dione), and only a slight activity toward testosterone to A-dione. Tumor-associated antigen in cutaneous T-cell lymphoma.
Alternative names
DHRS8, PAN1B, SDR16C2, PSEC0029, UNQ207/PRO233, HSD17B11, Estradiol 17-beta-dehydrogenase 11, 17-beta-hydroxysteroid dehydrogenase 11, 17-beta-hydroxysteroid dehydrogenase XI, Cutaneous T-cell lymphoma-associated antigen HD-CL-03, Dehydrogenase/reductase SDR family member 8, Retinal short-chain dehydrogenase/reductase 2, Short chain dehydrogenase/reductase family 16C member 2, 17-beta-HSD 11, 17bHSD11, 17betaHSD11, 17-beta-HSD XI, 17betaHSDXI, CTCL-associated antigen HD-CL-03, retSDR2
Recommended products
Rabbit Polyclonal Hsd17b11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B11 aa 200 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HSD17B11 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NBQ5
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsd17b11 also known as type 11 17-beta hydroxysteroid dehydrogenase is an enzyme that plays a role in steroid metabolism by catalyzing the conversion of less active 17-keto steroids to more active 17-beta-hydroxy steroids. The dehydrogenase weighs approximately 34 kDa and is found in various tissues but it is highly expressed in the liver and other steroidogenic tissues. As an NAD(P)-dependent oxidoreductase it contributes to maintaining steroid homeostasis within the cellular environment.
- Biological function summary
Hsd17b11 influences steroidal balance by regulating the levels of active and inactive steroid hormones. It is sometimes associated with lipid metabolism processes but remains independent not a part of any known enzymatic complex. By controlling hormone levels Hsd17b11 impacts cellular functions related to metabolism growth and reproduction reinforcing its importance in maintaining proper physiological conditions.
- Pathways
Hsd17b11 participates in steroid hormone metabolism and has roles within the broader steroidogenic pathway. It is related to the regulation of sex steroids closely working with enzymes like Hsd17b3 and Hsd17b2 which also modulate steroid hormones. These interactions contribute to comprehensive steroid biosynthesis and degradation pathways forming a network critical for endocrine system health.
- Associated diseases and disorders
Several studies have linked Hsd17b11 to disorders of steroid metabolism including polycystic ovary syndrome (PCOS) and some liver dysfunctions. Changes in its activity can affect steroid hormone levels perturbed in PCOS which often associates with aberrant androgen production. In metabolic liver disorders altered Hsd17b11 activity may disrupt lipid metabolism. The enzyme may also interact indirectly with proteins like SHBG (sex hormone-binding globulin) in these contexts influencing disease pathology and presentation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Hsd17b11 antibody (ab224437)
All lanes: Western blot - Anti-Hsd17b11 antibody (ab224437) at 1/100 dilution
All lanes: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsd17b11 antibody (ab224437)
Paraffin-embedded human colon tissue stained for Hsd17b11 using ab224437 at 1/20 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Hsd17b11 antibody (ab224437)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells stained for Hsd17b11 (green) using ab224437 at 4 μg/ml in ICC/IF.
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