Anti-HSD17B13 antibody [EPR28344-54]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] (AB316153)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling HSD17B13 with ab316153 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

MSD2027 control : no staining on human spleen. The section was incubated with ab316153 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] (AB316153)

Immunohistochemical analysis of paraffin-embedded Human fatty liver tissue labeling HSD17B13 with ab316153 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human fatty liver (PMID : 25028495). The section was incubated with ab316153 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] (AB316153)

Immunohistochemical analysis of paraffin-embedded Human normal liver tissue labeling HSD17B13 with ab316153 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human normal liver (PMID : 25028495). The section was incubated with ab316153 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-HSD17B13 antibody [EPR28344-54] (AB316153)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : thymus (PMID : 17311113), spleen (PMID : 17311113), tonsil.

The identity of the bands between 37 kDa and 75 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HSD17B13 antibody [EPR28344-54] (ab316153) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 50 µg

Lane 2:

Human thymus tissue lysate at 50 µg

Lane 3:

Human spleen tissue lysate at 50 µg

Lane 4:

Human tonsil tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 30 kDa,36 kDa

true

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-HSD17B13 antibody [EPR28344-54] (AB316153)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HepG2.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-HSD17B13 antibody [EPR28344-54] (ab316153) at 1/1000 dilution

Lane 1:

NCI-H69 (human lung small cell carcinoma cell) whole cell lysate at 40 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,124 kDa

false

Exposure time: 92s