Rabbit Recombinant Monoclonal HSD17B13 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426232.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426231.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426230.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154)")
![Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--western-blot-img426203.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154)")
![Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--western-blot-img426204.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (AB316154)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Plays a pivotal role in hepatic lipid metabolism (PubMed:29562163). In vitro, it catalyzes the oxidation of a variety of lipid substrates, including 17beta-estradiol, retinol, retinal, and leukotriene B4 (PubMed:29562163, PubMed:30415504, PubMed:32973038). Isoform 2. Has retinol/retinal dehydrogenase activity in vitro. Isoform 1. Does not have retinol/retinal dehydrogenase activity in vitro.
Alternative names
SCDR9, SDR16C3, HMFN0376, UNQ497/PRO1014, HSD17B13, 17-beta-hydroxysteroid dehydrogenase 13, 17-beta-HSD 13, Hepatic retinol/retinal dehydrogenase, Short chain dehydrogenase/reductase family 16C member 3, Short-chain dehydrogenase/reductase 9
Recommended products
Rabbit Recombinant Monoclonal HSD17B13 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28344-54
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316154 is the carrirer-free version of Anti-HSD17B13 antibody [EPR28344-54] ab316153.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426232.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)
This data was developed using Anti-HSD17B13 antibody [EPR28344-54] ab316153, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling HSD17B13 with Anti-HSD17B13 antibody [EPR28344-54] ab316153 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
MSD2027 control: no staining on human spleen. The section was incubated with Anti-HSD17B13 antibody [EPR28344-54] ab316153 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426231.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)
This data was developed using Anti-HSD17B13 antibody [EPR28344-54] ab316153, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human normal liver tissue labeling HSD17B13 with Anti-HSD17B13 antibody [EPR28344-54] ab316153 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human normal liver (PMID: 25028495). The section was incubated with Anti-HSD17B13 antibody [EPR28344-54] ab316153 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426230.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)
This data was developed using Anti-HSD17B13 antibody [EPR28344-54] ab316153, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human fatty liver tissue labeling HSD17B13 with Anti-HSD17B13 antibody [EPR28344-54] ab316153 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human fatty liver (PMID: 25028495). The section was incubated with Anti-HSD17B13 antibody [EPR28344-54] ab316153 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--western-blot-img426203.jpg "Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)")
Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)
This data was developed using Anti-HSD17B13 antibody [EPR28344-54] ab316153, the same antibody clone in a different buffer formulation.
Low expression: HepG2.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-HSD17B13 antibody [EPR28344-54] (Anti-HSD17B13 antibody [EPR28344-54] ab316153) at 1/1000 dilution
Lane 1: NCI-H69 (human lung small cell carcinoma cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa, 124 kDa
Exposure time: 92s
![Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154), expandable thumbnail](https://content.abcam.com/products/images/hsd17b13-antibody-epr28344-54-bsa-and-azide-free-ab316154--western-blot-img426204.jpg "Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)")
Western blot - Anti-HSD17B13 antibody [EPR28344-54] - BSA and Azide free (ab316154)
This data was developed using Anti-HSD17B13 antibody [EPR28344-54] ab316153, the same antibody clone in a different buffer formulation.
Negative control: thymus (PMID: 17311113), spleen (PMID: 17311113), tonsil.
The identity of the bands between 37 kDa and 75 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HSD17B13 antibody [EPR28344-54] (Anti-HSD17B13 antibody [EPR28344-54] ab316153) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 50 µg with 5% NFDM/TBST
Lane 2: Human thymus tissue lysate at 50 µg with 5% NFDM/TBST
Lane 3: Human spleen tissue lysate at 50 µg with 5% NFDM/TBST
Lane 4: Human tonsil tissue lysate at 50 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 30 kDa, 36 kDa
Exposure time: 92s
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