Rabbit Polyclonal HSD17B3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B3 aa 100-300.
Images
Publications
Filter by
- Systems biology in reproductive medicine 69:223-2332023Oxytocin modulates steroidogenesis-associated genes and estradiol levels in the placenta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung-Min An,Min Jae Kim,Jea Sic Jeong,So Young Kim,Da Som Kim,Beum-Soo An,Seung Chul KimPubMed 36787388
- Journal of cellular and molecular medicine 23:5956-59692019Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Chen et. al.PubMed 31293077
- Molecular medicine reports 19:3903-39112019Expression of steroidogenic enzymes in human placenta according to the gestational age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSo-Hye Hong et. al.PubMed 30896833
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HSD17B3 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link P37058
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids (PubMed:16216911, PubMed:26545797, PubMed:27927697, PubMed:8075637). Favors the reduction of androstenedione to testosterone (PubMed:16216911, PubMed:26545797, PubMed:27927697). Testosterone is the key androgen driving male development and function (PubMed:8075637). Uses NADPH while the two other EDH17B enzymes use NADH (PubMed:16216911, PubMed:26545797, PubMed:8075637). Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17 (PubMed:16216911). Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (PubMed:16216911, PubMed:27927697).
Alternative names
EDH17B3, SDR12C2, HSD17B3, 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, Estradiol 17-beta-dehydrogenase 2, Short chain dehydrogenase/reductase family 12C member 2, Testicular 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase 3
Recommended products
Rabbit Polyclonal HSD17B3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B3 aa 100-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HSD17B3 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link P37058
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-HSD17B3 antibody (ab126228)
12% SDS PAGE
All lanes: Western blot - Anti-HSD17B3 antibody (ab126228) at 1/500 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B3 antibody (ab126228)
ab126228, at a 1/500 dilution, staining HSD17B3 in paraffin embedded Hepatoma by Immunohistochemistry.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-HSD17B3 antibody (ab126228)
HSD17B3 western blot using anti-HSD17B3 antibody ab126228. Publication image and figure legend from Chen, Y., Li, C., et al., 2019, J Cell Mol Med, PubMed 31293077.
ab126228 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126228 please see the product overview.
Identification of Leydig‐like cells derived from induced pluripotent stem cells (ADSC‐LCs). A, Representative flow cytometry histograms for CYP17A1, HSD3B1, HSD11B1 and OCT4 in ADSCs, LCs and ADSC‐LCs. B, The quantitative histogram of flow cytometry assays. C, The measurement of biomark protein expressions of Leydig cells or ADSCs using Western blot in ADSCs, LCs and ADSC‐LCs
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com