AB126228

Anti-HSD17B3 antibody

Name: Anti-HSD17B3 antibody (ab126228) | Abcam
Brand: Abcam Limited
SKU: AB126228
Price: 520 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal HSD17B3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B3 aa 100-300.

View Alternative Names

EDH17B3, SDR12C2, HSD17B3, 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, Estradiol 17-beta-dehydrogenase 2, Short chain dehydrogenase/reductase family 12C member 2, Testicular 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase 3

4 Images

Supplier Data

Western blot - Anti-HSD17B3 antibody (AB126228)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-HSD17B3 antibody (ab126228) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-HSD17B3 antibody (AB126228)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-HSD17B3 antibody (ab126228) at 1/500 dilution

Lane 1:

Mouse testes tissue extracts at 50 µg

Lane 2:

Rat testes tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B3 antibody (AB126228)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B3 antibody (AB126228)

ab126228, at a 1/500 dilution, staining HSD17B3 in paraffin embedded Hepatoma by Immunohistochemistry.

CiteAb

Western blot - Anti-HSD17B3 antibody (AB126228)

HSD17B3 western blot using anti-HSD17B3 antibody ab126228. Publication image and figure legend from Chen, Y., Li, C., et al., 2019, J Cell Mol Med, PubMed 31293077.

ab126228 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126228 please see the product overview.

Identification of Leydig‐like cells derived from induced pluripotent stem cells (ADSC‐LCs). A, Representative flow cytometry histograms for CYP17A1, HSD3B1, HSD11B1 and OCT4 in ADSCs, LCs and ADSC‐LCs. B, The quantitative histogram of flow cytometry assays. C, The measurement of biomark protein expressions of Leydig cells or ADSCs using Western blot in ADSCs, LCs and ADSC‐LCs

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HSD17B3 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P37058

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids (PubMed : 16216911, PubMed : 26545797, PubMed : 27927697, PubMed : 8075637). Favors the reduction of androstenedione to testosterone (PubMed : 16216911, PubMed : 26545797, PubMed : 27927697). Testosterone is the key androgen driving male development and function (PubMed : 8075637). Uses NADPH while the two other EDH17B enzymes use NADH (PubMed : 16216911, PubMed : 26545797, PubMed : 8075637). Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17 (PubMed : 16216911). Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (PubMed : 16216911, PubMed : 27927697).

See full target information HSD17B3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1396890 PubMed38983788

2024

Screening of potentially active compounds against rheumatoid arthritis in the Juan-Bi decoction using systems pharmacology and animal experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Dahai Liu,Qiang Fu,Leyna G Liu,Wenwen Li,Fei Qi,Justin Liu,Lifeng Shang,Xiu Wang,Fang Yang,Jie Li,Daoqiang Lu,Huiying Feng,Ziwen Zhang,Yiqing Chen,Junru Liang,Jiayi Yao,Hua Lv,Riwang Li,Jun Wang,Di Wu,Yuxi Liu,Chenglai Xia,Wenxing Li

Systems biology in reproductive medicine 69:223-233 PubMed36787388

2023

Oxytocin modulates steroidogenesis-associated genes and estradiol levels in the placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Min An,Min Jae Kim,Jea Sic Jeong,So Young Kim,Da Som Kim,Beum-Soo An,Seung Chul Kim

Molecular and cellular endocrinology 542:111519 PubMed34843900

2021

GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis.

Applications

Unspecified application

Species

Unspecified reactive species

Matheus Felipe Fonseca Gonçalves,Samyra Maria Dos Santos Nassif Lacerda,Nathália de Lima E Martins Lara,Carolina Felipe Alves de Oliveira,André Felipe Almeida Figueiredo,Marcos Rocha Gouvêa Brener,Marina Alcântara Cavalcante,Anderson Kenedy Santos,Gabriel Henrique Campolina-Silva,Vivian Vasconcelos Costa,Ana Clara Campideli Santana,Roberta Araújo Lopes,Raphael Escorsim Szawka,Guilherme Mattos Jardim Costa

Journal of cellular and molecular medicine 23:5956-5969 PubMed31293077

2019

Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Chen,Chao Li,Weiping Ji,Long Wang,Xianwu Chen,Shenzhi Zhao,Zhangye Xu,Renshan Ge,Xiaoling Guo

Molecular medicine reports 19:3903-3911 PubMed30896833

2019

Expression of steroidogenic enzymes in human placenta according to the gestational age.

Applications

Unspecified application

Species

Unspecified reactive species

So-Hye Hong,Seung Chul Kim,Mee-Na Park,Jea Sic Jeong,Seung Yun Yang,Young Joo Lee,Ok-Nam Bae,Hoe-Saeng Yang,Sungbaek Seo,Kyu-Sup Lee,Beum-Soo An

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HSD17B3 antibody

Western blot - Anti-HSD17B3 antibody (AB126228)

Western blot - Anti-HSD17B3 antibody (AB126228)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B3 antibody (AB126228)

Western blot - Anti-HSD17B3 antibody (AB126228)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise